Dataset Information

First Report of the Lesion Nematodes: Pratylenchus Brachyurus and Pratylenchus Delattrei on Tomato (Solanum Lycopersicum L.) Plants in Cape Verde.

ABSTRACT: Roots of Solanum lycopersicum L. were collected in growing season of year 2015, on the island of Santiago in Cape Verde. Morphological, morphometric and molecular (18S rDNA and 28S rDNA) studies revealed the presence of Pratylenchus brachyurus and P. delattrei in root systems and root zones of tomato plants. To our knowledge, this is the first record of the occurrence of these nematode species in Cape Verde.

SUBMITTER: Flis L

PROVIDER: S-EPMC6799531 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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First Report of the Lesion Nematodes: Pratylenchus Brachyurus and Pratylenchus Delattrei on Tomato (Solanum Lycopersicum L.) Plants in Cape Verde.

Flis Ł Ł Dobosz R R Rybarczyk-Mydłowska K K Wasilewska-Nascimento B B Kubicz M M Winiszewska G G

Helminthologia 20180127 1

Roots of Solanum lycopersicum L. were collected in growing season of year 2015, on the island of Santiago in Cape Verde. Morphological, morphometric and molecular (18S rDNA and 28S rDNA) studies revealed the presence of Pratylenchus brachyurus and P. delattrei in root systems and root zones of tomato plants. To our knowledge, this is the first record of the occurrence of these nematode species in Cape Verde. ...[more]

PMID: 31662632

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Project description:BackgroundEndogenous pararetroviral sequences (EPRVs) are a recently discovered class of repetitive sequences that is broadly distributed in the plant kingdom. The potential contribution of EPRVs to plant pathogenicity or, conversely, to virus resistance is just beginning to be explored. Some members of the family Solanaceae are particularly rich in EPRVs. In previous work, EPRVs have been characterized molecularly in various species of Nicotiana including N.tabacum (tobacco) and Solanum tuberosum (potato). Here we describe a family of EPRVs in cultivated tomato (Solanum lycopersicum L.) and a wild relative (S.habrochaites).ResultsMolecular cloning and DNA sequence analysis revealed that tomato EPRVs (named LycEPRVs) are most closely related to those in tobacco. The sequence similarity of LycEPRVs in S.lycopersicum and S.habrochaites indicates they are potentially derived from the same pararetrovirus. DNA blot analysis revealed a similar genomic organization in the two species, but also some independent excision or insertion events after species separation, or flanking sequence divergence. LycEPRVs share with the tobacco elements a disrupted genomic structure and frequent association with retrotransposons. Fluorescence in situ hybridization revealed that copies of LycEPRV are dispersed on all chromosomes in predominantly heterochromatic regions. Methylation of LycEPRVs was detected in CHG and asymmetric CHH nucleotide groups. Although normally quiescent EPRVs can be reactivated and produce symptoms of infection in some Nicotiana interspecific hybrids, a similar pathogenicity of LycEPRVs could not be demonstrated in Solanum L. section Lycopersicon [Mill.] hybrids. Even in healthy plants, however, transcripts derived from multiple LycEPRV loci and short RNAs complementary to LycEPRVs were detected and were elevated upon infection with heterologous viruses encoding suppressors of PTGS.ConclusionThe analysis of LycEPRVs provides further evidence for the extensive invasion of pararetroviral sequences into the genomes of solanaceous plants. The detection of asymmetric CHH methylation and short RNAs, which are hallmarks of RNAi in plants, suggests that LycEPRVs are controlled by an RNA-mediated silencing mechanism.

Dataset Information

First Report of the Lesion Nematodes: Pratylenchus Brachyurus and Pratylenchus Delattrei on Tomato (Solanum Lycopersicum L.) Plants in Cape Verde.

Publications

First Report of the Lesion Nematodes: <i>Pratylenchus Brachyurus</i> and <i>Pratylenchus Delattrei</i> on Tomato (<i>Solanum Lycopersicum</i> L.) Plants in Cape Verde.

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