Dataset Information

Integrated transcriptomic and secretomic approaches reveal critical pathogenicity factors in Pseudofabraea citricarpa inciting citrus target spot.

ABSTRACT: Target spot is a newly emerging citrus disease caused by Pseudofabraea citricarpa. Outbreaks of this disease result in massive economic losses to citrus production. Here, an integrated study involving comparative transcriptomic and secretomic analyses was conducted to determine the critical pathogenicity factors of P. citricarpa involved in the induction of citrus target spot. A total of 701 transcripts and their cognate proteins were quantified and integrated. Among these transcripts and proteins, 99 exhibited the same expression patterns. Our quantitative integrated multi-omic data highlight several potentially pivotal pathogenicity factors, including 16 unigenes that were annotated as plant cell-wall-degrading enzymes, 13 unigenes homologous to virulence factors from various fungi, and one unigene described as a small cysteine-rich secreted protein, were screened and analysed. The screening of differentially expressed genes that encode secondary metabolism core enzymes implicated terpene metabolism in the pathogenicity of P. citricarpa. Overall, results indicated that plant cell wall degradation, plant-pathogen protein/polyribonucleotide interaction, and terpene biosynthesis have critical roles in the pathogenicity of P. citricarpa. This work demonstrated that integrated omic approaches enable the identification of pathogenicity/virulence factors and provide insights into the mechanisms underlying the pathogenicity of fungi. These insights would aid the development of effective disease management strategies.

SUBMITTER: Yang Y

PROVIDER: S-EPMC6801157 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Integrated transcriptomic and secretomic approaches reveal critical pathogenicity factors in Pseudofabraea citricarpa inciting citrus target spot.

Yang Yuheng Y Fang Anfei A Yu Yang Y Bi Chaowei C Zhou Changyong C

Microbial biotechnology 20190604 6

Target spot is a newly emerging citrus disease caused by Pseudofabraea citricarpa. Outbreaks of this disease result in massive economic losses to citrus production. Here, an integrated study involving comparative transcriptomic and secretomic analyses was conducted to determine the critical pathogenicity factors of P. citricarpa involved in the induction of citrus target spot. A total of 701 transcripts and their cognate proteins were quantified and integrated. Among these transcripts and protei ...[more]

PMID: 31162831

Similar Datasets

Project description:Several Phyllosticta species are known as pathogens of Citrus spp., and are responsible for various disease symptoms including leaf and fruit spots. One of the most important species is P. citricarpa, which causes a foliar and fruit disease called citrus black spot. The Phyllosticta species occurring on citrus can most effectively be distinguished from P. citricarpa by means of multilocus DNA sequence data. Recent studies also demonstrated P. citricarpa to be heterothallic, and reported successful mating in the laboratory. Since the domestication of citrus, different clones of P. citricarpa have escaped Asia to other continents via trade routes, with obvious disease management consequences. This pathogen profile represents a comprehensive literature review of this pathogen and allied taxa associated with citrus, focusing on identification, distribution, genomics, epidemiology and disease management. This review also considers the knowledge emerging from seven genomes of Phyllosticta spp., demonstrating unknown aspects of these species, including their mating behaviour.TaxonomyPhyllosticta citricarpa (McAlpine) Aa, 1973. Kingdom Fungi, Phylum Ascomycota, Class Dothideomycetes, Order Botryosphaeriales, Family Phyllostictaceae, Genus Phyllosticta, Species citricarpa.Host rangeConfirmed on more than 12 Citrus species, Phyllosticta citricarpa has only been found on plant species in the Rutaceae.Disease symptomsP. citricarpa causes diverse symptoms such as hard spot, virulent spot, false melanose and freckle spot on fruit, and necrotic lesions on leaves and twigs.Useful websitesDOE Joint Genome Institute MycoCosm portals for the Phyllosticta capitalensis (https://genome.jgi.doe.gov/Phycap1), P. citriasiana (https://genome.jgi.doe.gov/Phycit1), P. citribraziliensis (https://genome.jgi.doe.gov/Phcit1), P. citrichinaensis (https://genome.jgi.doe.gov/Phcitr1), P. citricarpa (https://genome.jgi.doe.gov/Phycitr1, https://genome.jgi.doe.gov/Phycpc1), P. paracitricarpa (https://genome.jgi.doe.gov/Phy27169) genomes. All available Phyllosticta genomes on MycoCosm can be viewed at https://genome.jgi.doe.gov/Phyllosticta.

Project description:Juice sac granulation is a common internal physiological disorder of citrus fruit. In the present study, we compared the physiochemical characteristics and transcriptome profiles of juice sacs in different granulation levels from Huyou fruit (Citrus changshanensis). The accumulation of cell wall components, including the water-soluble pectin, protopectin, cellulose, and lignin, were significantly correlated with the granulation process, resulting in the firmness increase of the juice sac. The in situ labeling of the cell wall components indicated the early accumulation of cellulose and high-methylesterified pectin in the outer layer cells, as well as the late accumulation of lignin in the inner layer cells of the juice sac. Several phytohormones, including auxins, abscisic acids, cytokinins, jasmonic acid, salicylic acid, and/or their metabolites, were positively correlated to the granulation level, indicating an active and complex phytohormones metabolism in the granulation process. Combining the trend analysis by the Mfuzz method and the module-trait correlation analysis by the Weighted Gene Co-expression Network Analysis method, a total of 2940 differentially expressed genes (DEGs) were found to be positively correlated with the granulation level. Gene Ontology (GO) enrichment indicated that the selected DEGs were mainly involved in the cell wall organization and biogenesis, cell wall macromolecule metabolic process, carbohydrate metabolic process, and polysaccharide metabolic process. Among these selected genes, those encoding β-1,4-xylosyltransferase IRX9, cellulose synthase, xyloglucan: xyloglucosyl transferase, xyloglucan galactosyltransferase MUR3, α-1,4-galacturonosyltransferase, expansin, polygalacturonase, pectinesterase, β-glucosidase, β-galactosidase, endo-1,3(4)-β-glucanase, endoglucanase and pectate lyase that required for the biosynthesis or structural modification of cell wall were identified. In addition, NAC, MYB, bHLH, and MADS were the top abundant transcription factors (TFs) families positively correlated with the granulation level, while the LOB was the top abundant TFs family negatively correlated with the granulation level.

Project description:Brown spot of citrus caused by Alternaria citri is one of the emerging threats to the successful production of citrus crops. The present study, conducted with a substantial sample size of 50 leaf samples for statistical reliability, aimed to determine the change in mineral content in citrus leaves after brown spot disease attack. Leaf samples from a diverse range of susceptible citrus varieties (Valentia late, Washington navel, and Kinnow) and resistant varieties (Citron, Eruka lemon, and Mayer lemon) were analyzed. Significant variations (p ≤ 0.05) in mineral contents were observed across reaction groups (inoculated and un-inoculated), types (resistant and susceptible), and varieties of citrus in response to infection of Alternaria citri. The analysis of variance showed significant changes in mineral levels of citrus leaves, including nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), zinc (Zn), sodium (Na), iron (Fe), and copper (Cu). The results indicate that the concentration of N and P differed by 6.63% and 1.44%, respectively, in resistant plants, while susceptible plants showed a difference of 6.07% and 1.19%. Moreover, resistant plants showed a higher concentrations of K, Ca, Mg, Zn, Na, Fe, and Cu at 8.40, 2.1, 1.83, 2.21, 1.58, 2.89, and 0.36 ppm respectively, compared to susceptible plants which showed concentrations of 5.99, 1.93, 1.47, 1.09, 1.24, 1.81, and 0.31 ppm respectively. Amounts of mineral contents were reduced in both resistant as well as susceptible plants of citrus after inoculation. Amount of N (8.56), P (1.87) % while K (10.74), Ca (2.71), Mg (2.62), Zn (2.20), Na (2.08), Fe (3.57) and Cu (0.20) ppm were recorded in un-inoculated group of citrus plants that reduced to 3.15 and 0.76% and 3.66, 1.40, 0.63,0.42, 0.74, 1.13 and 0.13 ppm in inoculated group respectively. It was accomplished that susceptible varieties contained lower ionic contents than resistant varieties. The higher concentrations of ionic contents in resistant citrus varieties build up the biochemical and physiological processes of the citrus plant, which help to restrict spread of pathogens. Further research could explore the interplay between mineral nutrition and disease resistance in citrus, potentially leading to the development of new disease-resistant varieties.

Dataset Information

Integrated transcriptomic and secretomic approaches reveal critical pathogenicity factors in Pseudofabraea citricarpa inciting citrus target spot.

Publications

Integrated transcriptomic and secretomic approaches reveal critical pathogenicity factors in Pseudofabraea citricarpa inciting citrus target spot.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets