Ontology highlight
ABSTRACT:
SUBMITTER: Itoh Y
PROVIDER: S-EPMC6802517 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Itoh Yuka Y Koinuma Daizo D Omata Chiho C Ogami Tomohiro T Motizuki Mitsuyoshi M Yaguchi So-Ichi SI Itoh Takuma T Miyake Kunio K Tsutsumi Shuichi S Aburatani Hiroyuki H Saitoh Masao M Miyazono Kohei K Miyazawa Keiji K
The Journal of biological chemistry 20190903 42
Smad proteins are transcriptional regulators activated by TGF-β. They are known to bind to two distinct Smad-responsive motifs, namely the Smad-binding element (SBE) (5'-GTCTAGAC-3') and CAGA motifs (5'-AGCCAGACA-3' or 5'-TGTCTGGCT-3'). However, the mechanisms by which these motifs promote Smad activity are not fully elucidated. In this study, we performed DNA CASTing, binding assays, ChIP sequencing, and quantitative RT-PCR to dissect the details of Smad binding and function of the SBE and CAGA ...[more]