Ontology highlight
ABSTRACT:
SUBMITTER: Zhou D
PROVIDER: S-EPMC6803305 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Applied and environmental microbiology 20191016 21
<i>Lactobacillus plantarum</i> is a potential starter and health-promoting probiotic bacterium. Effective, precise, and diverse genome editing of <i>Lactobacillus plantarum</i> without introducing exogenous genes or plasmids is of great importance. In this study, CRISPR/Cas9-assisted double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) recombineering was established in <i>L. plantarum</i> WCFS1 to seamlessly edit the genome, including gene knockouts, insertions, and point mutations. To op ...[more]