Ontology highlight
ABSTRACT:
SUBMITTER: Arias-Fuenzalida J
PROVIDER: S-EPMC5830965 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Arias-Fuenzalida Jonathan J Jarazo Javier J Qing Xiaobing X Walter Jonas J Gomez-Giro Gemma G Nickels Sarah Louise SL Zaehres Holm H Schöler Hans Robert HR Schwamborn Jens Christian JC
Stem cell reports 20171005 5
Genome editing and human induced pluripotent stem cells hold great promise for the development of isogenic disease models and the correction of disease-associated mutations for isogenic tissue therapy. CRISPR-Cas9 has emerged as a versatile and simple tool for engineering human cells for such purposes. However, the current protocols to derive genome-edited lines require the screening of a great number of clones to obtain one free of random integration or on-locus non-homologous end joining (NHEJ ...[more]