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Ratiometric Polymer Probe for Detection of Peroxynitrite and the Application for Live-Cell Imaging.

ABSTRACT: Peroxynitrite (ONOO-) is one of the sources of oxidation stress involved in many biological signaling pathways. The role of ONOO- being a double-edged sword in biological systems drives the development of effective detection tools. In this work, a boronate-based polymeric fluorescent probe PB-PVA was synthesized and the probe performance was evaluated. The probe exhibits ratiometric sensing of ONOO- in a range of 0-6 µM. There is good linear relationship between the probe fluorescence intensity ratio and ONOO- concentration. The probe also displays moderate selectivity towards ONOO- over other ROS. Moreover, it is water-soluble and possesses good biocompatibility which aids the imaging of ONOO- in living cells. These properties could make the probe a promising tool in in vitro study related to ONOO-.

SUBMITTER: Lao HK

PROVIDER: S-EPMC6804088 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Ratiometric Polymer Probe for Detection of Peroxynitrite and the Application for Live-Cell Imaging.

Lao Hio Kuan HK Tan Jingyun J Wang Chunfei C Zhang Xuanjun X

Molecules (Basel, Switzerland) 20190924 19

Peroxynitrite (ONOO-) is one of the sources of oxidation stress involved in many biological signaling pathways. The role of ONOO- being a double-edged sword in biological systems drives the development of effective detection tools. In this work, a boronate-based polymeric fluorescent probe PB-PVA was synthesized and the probe performance was evaluated. The probe exhibits ratiometric sensing of ONOO- in a range of 0-6 µM. There is good linear relationship between ...[more]

PMID: 31554286

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