Dataset Information

Draft genome sequences of two oriental melons, Cucumis melo L. var. makuwa.

ABSTRACT: Oriental melon (Cucumis melo L. var. makuwa) is one of the most important cultivated cucurbits, and is grown widely in Northeast Asian countries. With increasing interest in its biological properties and economic importance, oriental melon has become an attractive model crop for studying various horticultural traits. A previous genome sequence of the melon was constructed from a homozygous double-haploid line. Thus, individual reference genomes are required to perform functional studies and further breeding applications. Here, we report draft genome sequences of two oriental melons, Chang Bougi and SW3. The assembled 344?Mb genome of Chang Bougi was obtained with scaffold N50 1.0?Mb, and 36,235 genes were annotated. The 354?Mb genome of SW3 was assembled with scaffold N50 1.6?Mb, and has 38,173 genes. These newly constructed genomes will enable studies of fruit development, disease resistance, and breeding applications in the oriental melon.

SUBMITTER: Shin AY

PROVIDER: S-EPMC6805853 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Draft genome sequences of two oriental melons, Cucumis melo L. var. makuwa.

Shin Ah-Young AY Koo Namjin N Kim Seungill S Sim Young Mi YM Choi Doil D Kim Yong-Min YM Kwon Suk-Yoon SY

Scientific data 20191022 1

Oriental melon (Cucumis melo L. var. makuwa) is one of the most important cultivated cucurbits, and is grown widely in Northeast Asian countries. With increasing interest in its biological properties and economic importance, oriental melon has become an attractive model crop for studying various horticultural traits. A previous genome sequence of the melon was constructed from a homozygous double-haploid line. Thus, individual reference genomes are required to perform functional studies and furt ...[more]

PMID: 31641135

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Project description:BACKGROUND:Lipoxygenases (LOXs) play significant roles in abiotic stress responses, and identification of LOX gene promoter function can make an important contribution to elucidating resistance mechanisms. Here, we cloned the CmLOX08 promoter of melon (Cucumis melo) and identified the main promoter regions regulating transcription in response to signalling molecules and abiotic stresses. RESULTS:The 2054-bp promoter region of CmLOX08 from melon leaves was cloned, and bioinformatic analysis revealed that it harbours numerous cis-regulatory elements associated with signalling molecules and abiotic stress. Five 5'-deletion fragments obtained from the CmLOX08 promoter-2054 (LP1), 1639 (LP2), 1284 (LP3), 1047 (LP4), and 418?bp (LP5)-were fused with a GUS reporter gene and used for tobacco transient assays. Deletion analysis revealed that in response to abscisic acid, salicylic acid, and hydrogen peroxide, the GUS activity of LP1 was significantly higher than that of the mock-treated control and LP2, indicating that the -?2054- to -?1639-bp region positively regulates expression induced by these signalling molecules. However, no deletion fragment GUS activity was induced by methyl jasmonate. In response to salt, drought, and wounding treatments, LP1, LP2, and LP4 promoted significantly higher GUS expression compared with the control. Among all deletion fragments, LP4 showed the highest GUS expression, indicating that -?1047 to -?1?bp is the major region regulating promoter activity and that the -?1047 to -?418-bp region positively regulates expression induced by salt, drought, and wounding, whereas the -?1284 to -?1047-bp region is a negative regulatory segment. Interestingly, although the GUS activity of LP1 and LP2 was not affected by temperature changes, that of LP3 was significantly induced by heat, indicating that the -?1284- to -?1-bp region is a core sequence responding to heat and the -?2054- to -?1284-bp region negatively regulates expression induced by heat. Similarly, the -?1047- to -?1-bp region is the main sequence responding to cold, whereas the -?2054- to -?1047-bp region negatively regulates expression induced by cold. CONCLUSIONS:We cloned the CmLOX08 promoter and demonstrated that it is a signalling molecule/stress-inducible promoter. Furthermore, we identified core and positive/negative regulatory regions responding to three signalling molecules and five abiotic stresses.

Dataset Information

Draft genome sequences of two oriental melons, Cucumis melo L. var. makuwa.

Publications

Draft genome sequences of two oriental melons, Cucumis melo L. var. makuwa.

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