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Prevalence and spectrum of MLH1, MSH2, and MSH6 pathogenic germline variants in Pakistani colorectal cancer patients.


ABSTRACT: Background:Pathogenic germline variants in MLH1, MSH2 and MSH6 genes account for the majority of Lynch syndrome (LS). In this first report from Pakistan, we investigated the prevalence of pathogenic MLH1/MSH2/MSH6 variants in colorectal cancer (CRC) patients. Methods:Consecutive cases (n?=?212) were recruited at the Shaukat Khanum Memorial Cancer Hospital and Research Centre (SKMCH&RC), between November 2007 to March 2011. Patients with a family history of >?3 or 2 HNPCC-associated cancers were classified as HNPCC (n?=?9) or suspected-HNPCC (n?=?20), respectively (group 1; n?=?29). Cases with no family history were designated as non-HNPCC (group 2; n?=?183). MLH1/MSH2/MSH6 genes were comprehensively screened in group 1. Pathogenic/likely pathogenic variants identified in group 1 were subsequently evaluated in group 2. Results:Eight distinct pathogenic/likely pathogenic MLH1/MSH2 variants were found in group 1 (10/29; 34.5%), belonging to HNPCC (5/9; 55.6%) and suspected-HNPCC (5/20; 25%) families and in group 2 (2/183; 1.1%) belonging to non-HNPCC. Overall, three recurrent variants (MSH2 c.943-1G?>?C, MLH1 c.1358dup and c.2041G?>?A) accounted for 58.3% (7/12) of all families harboring pathogenic/likely pathogenic MLH1/MSH2 variants. Pathogenic MSH6 variants were not detected. Conclusion:Pathogenic/likely pathogenic MLH1/MSH2 variants account for a substantial proportion of CRC patients with HNPCC/suspected-HNPCC in Pakistan. Our findings suggest that HNPCC/suspected-HNPCC families should be tested for these recurrent variants prior to comprehensive gene screening in this population.

SUBMITTER: Rashid MU 

PROVIDER: S-EPMC6806584 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Prevalence and spectrum of <i>MLH1</i>, <i>MSH2</i>, and <i>MSH6</i> pathogenic germline variants in Pakistani colorectal cancer patients.

Rashid Muhammad Usman MU   Naeemi Humaira H   Muhammad Noor N   Loya Asif A   Lubiński Jan J   Jakubowska Anna A   Yusuf Muhammed Aasim MA  

Hereditary cancer in clinical practice 20191023


<h4>Background</h4>Pathogenic germline variants in <i>MLH1</i>, <i>MSH2</i> and <i>MSH6</i> genes account for the majority of Lynch syndrome (LS). In this first report from Pakistan, we investigated the prevalence of pathogenic <i>MLH1/MSH2/MSH6</i> variants in colorectal cancer (CRC) patients.<h4>Methods</h4>Consecutive cases (<i>n</i> = 212) were recruited at the Shaukat Khanum Memorial Cancer Hospital and Research Centre (SKMCH&RC), between November 2007 to March 2011. Patients with a family  ...[more]

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