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An in vitro platform for elucidating the molecular genetics of S. aureus invasion of the osteocyte lacuno-canalicular network during chronic osteomyelitis.

ABSTRACT: Staphylococcus aureus osteomyelitis is a devasting disease that often leads to amputation. Recent findings have shown that S. aureus is capable of invading the osteocyte lacuno-canalicular network (OLCN) of cortical bone during chronic osteomyelitis. Normally a 1??m non-motile cocci, S. aureus deforms smaller than 0.5??m in the sub-micron channels of the OLCN. Here we present the ?SiM-CA (Microfluidic - Silicon Membrane - Canalicular Array) as an in vitro screening platform for the genetic mechanisms of S. aureus invasion. The ?SiM-CA platform features an ultrathin silicon membrane with defined pores that mimic the openings of canaliculi. While we anticipated that S. aureus lacking the accessory gene regulator (agr) quorum-sensing system would not be capable of invading the OLCN, we found no differences in propagation compared to wild type in the ?SiM-CA. However the ?SiM-CA proved predictive as we also found that the agr mutant strain invaded the OLCN of murine tibiae.

SUBMITTER: Masters EA

PROVIDER: S-EPMC6814543 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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An in vitro platform for elucidating the molecular genetics of S. aureus invasion of the osteocyte lacuno-canalicular network during chronic osteomyelitis.

Masters Elysia A EA Salminen Alec T AT Begolo Stefano S Luke Emma N EN Barrett Sydney C SC Overby Clyde T CT Gill Ann Lindley AL de Mesy Bentley Karen L KL Awad Hani A HA Gill Steven R SR Schwarz Edward M EM McGrath James L JL

Nanomedicine : nanotechnology, biology, and medicine 20190624

Staphylococcus aureus osteomyelitis is a devasting disease that often leads to amputation. Recent findings have shown that S. aureus is capable of invading the osteocyte lacuno-canalicular network (OLCN) of cortical bone during chronic osteomyelitis. Normally a 1 μm non-motile cocci, S. aureus deforms smaller than 0.5 μm in the sub-micron channels of the OLCN. Here we present the μSiM-CA (Microfluidic - Silicon Membrane - Canalicular Array) as an in vitro screening platform for the genetic mecha ...[more]

PMID: 31247310

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Dataset Information

An in vitro platform for elucidating the molecular genetics of S. aureus invasion of the osteocyte lacuno-canalicular network during chronic osteomyelitis.

Publications

An in vitro platform for elucidating the molecular genetics of S. aureus invasion of the osteocyte lacuno-canalicular network during chronic osteomyelitis.

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