Peptide Deformylase (def) is essential in Mycobacterium smegmatis, but the essentiality is compensated by inactivation of methionine formylation.
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ABSTRACT:
SUBMITTER: Naor N
PROVIDER: S-EPMC6815462 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Publications
Peptide Deformylase (def) is essential in Mycobacterium smegmatis, but the essentiality is compensated by inactivation of methionine formylation.
Naor Noga N Gadot Omer O Meir Michal M Barkan Daniel D
BMC microbiology 20191026 1
<h4>Background</h4>Co-translational processes in bacteria are attractive drug targets, but while some processes are essential, others are not. The essentiality of Peptide Deformylase (PDF, def) for vitality of mycobacteria was speculated, but never unequivocally proven.<h4>Results</h4>Here we show by targeted deletion experiments that def can only be deleted from M. smegmatis when an additional copy is present; that prior deletion of tRNA<sup>fMet</sup>-Formyl Transferase (FMT, encoded by fmt) r ...[more]
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