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Multicountry Distribution and Characterization of Extended-spectrum ?-Lactamase-associated Gram-negative Bacteria From Bloodstream Infections in Sub-Saharan Africa.


ABSTRACT: BACKGROUND:Antimicrobial resistance (AMR) is a major global health concern, yet, there are noticeable gaps in AMR surveillance data in regions such as sub-Saharan Africa. We aimed to measure the prevalence of extended-spectrum ?-lactamase (ESBL) producing Gram-negative bacteria in bloodstream infections from 12 sentinel sites in sub-Saharan Africa. METHODS:Data were generated during the Typhoid Fever Surveillance in Africa Program (TSAP), in which standardized blood cultures were performed on febrile patients attending 12 health facilities in 9 sub-Saharan African countries between 2010 and 2014. Pathogenic bloodstream isolates were identified at the sites and then subsequently confirmed at a central reference laboratory. Antimicrobial susceptibility testing, detection of ESBL production, and conventional multiplex polymerase chain reaction (PCR) testing for genes encoding for ?-lactamase were performed on all pathogens. RESULTS:Five hundred and five pathogenic Gram-negative bloodstream isolates were isolated during the study period and available for further characterization. This included 423 Enterobacteriaceae. Phenotypically, 61 (12.1%) isolates exhibited ESBL activity, and genotypically, 47 (9.3%) yielded a PCR amplicon for at least one of the screened ESBL genes. Among specific Gram-negative isolates, 40 (45.5%) of 88 Klebsiella spp., 7 (5.7%) of 122 Escherichia coli, 6 (16.2%) of 37 Acinetobacter spp., and 2 (1.3%) of 159 of nontyphoidal Salmonella (NTS) showed phenotypic ESBL activity. CONCLUSIONS:Our findings confirm the presence of ESBL production among pathogens causing bloodstream infections in sub-Saharan Africa. With few alternatives for managing ESBL-producing pathogens in the African setting, measures to control the development and proliferation of AMR organisms are urgently needed.

SUBMITTER: Toy T 

PROVIDER: S-EPMC6821266 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Multicountry Distribution and Characterization of Extended-spectrum β-Lactamase-associated Gram-negative Bacteria From Bloodstream Infections in Sub-Saharan Africa.

Toy Trevor T   Pak Gi Deok GD   Duc Trung Pham TP   Campbell James I JI   El Tayeb Muna Ahmed MA   Von Kalckreuth Vera V   Im Justin J   Panzner Ursula U   Cruz Espinoza Ligia Maria LM   Eibach Daniel D   Dekker Denise Myriam DM   Park Se Eun SE   Jeon Hyon Jin HJ   Konings Frank F   Mogeni Ondari D OD   Cosmas Leonard L   Bjerregaard-Andersen Morten M   Gasmelseed Nagla N   Hertz Julian T JT   Jaeger Anna A   Krumkamp Ralf R   Ley Benedikt B   Thriemer Kamala K   Kabore Leon Parfait LP   Niang Aissatou A   Raminosoa Tiana Mirana TM   Sampo Emmanuel E   Sarpong Nimako N   Soura Abdramane A   Owusu-Dabo Ellis E   Teferi Mekonnen M   Yeshitela Biruk B   Poppert Sven S   May Jürgen J   Kim Jerome H JH   Chon Yun Y   Park Jin Kyung JK   Aseffa Abroaham A   Breiman Robert F RF   Schütt-Gerowitt Heidi H   Aaby Peter P   Adu-Sarkodie Yaw Y   Crump John A JA   Rakotozandrindrainy Raphaël R   Meyer Christian G CG   Sow Amy Gassama AG   Clemens John D JD   Wierzba Thomas F TF   Baker Stephen S   Marks Florian F  

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 20191001 Suppl 6


<h4>Background</h4>Antimicrobial resistance (AMR) is a major global health concern, yet, there are noticeable gaps in AMR surveillance data in regions such as sub-Saharan Africa. We aimed to measure the prevalence of extended-spectrum β-lactamase (ESBL) producing Gram-negative bacteria in bloodstream infections from 12 sentinel sites in sub-Saharan Africa.<h4>Methods</h4>Data were generated during the Typhoid Fever Surveillance in Africa Program (TSAP), in which standardized blood cultures were  ...[more]

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