Ontology highlight
ABSTRACT:
SUBMITTER: Riesenberg S
PROVIDER: S-EPMC6821318 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Riesenberg Stephan S Chintalapati Manjusha M Macak Dominik D Kanis Philipp P Maricic Tomislav T Pääbo Svante S
Nucleic acids research 20191101 19
When double-strand breaks are introduced in a genome by CRISPR they are repaired either by non-homologous end joining (NHEJ), which often results in insertions or deletions (indels), or by homology-directed repair (HDR), which allows precise nucleotide substitutions to be introduced if a donor oligonucleotide is provided. Because NHEJ is more efficient than HDR, the frequency with which precise genome editing can be achieved is so low that simultaneous editing of more than one gene has hitherto ...[more]