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Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension.


ABSTRACT: Clathrin light chains (CLCa and CLCb) are major constituents of clathrin-coated vesicles. Unique functions for these evolutionary conserved paralogs remain elusive, and their role in clathrin-mediated endocytosis in mammalian cells is debated. Here, we find and structurally characterize a direct and selective interaction between CLCa and the long isoform of the actin motor protein myosin VI, which is expressed exclusively in highly polarized tissues. Using genetically-reconstituted Caco-2 cysts as proxy for polarized epithelia, we provide evidence for coordinated action of myosin VI and CLCa at the apical surface where these proteins are essential for fission of clathrin-coated pits. We further find that myosin VI and Huntingtin-interacting protein 1-related protein (Hip1R) are mutually exclusive interactors with CLCa, and suggest a model for the sequential function of myosin VI and Hip1R in actin-mediated clathrin-coated vesicle budding.

SUBMITTER: Biancospino M 

PROVIDER: S-EPMC6823378 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension.

Biancospino Matteo M   Buel Gwen R GR   Niño Carlos A CA   Maspero Elena E   Scotto di Perrotolo Rossella R   Raimondi Andrea A   Redlingshöfer Lisa L   Weber Janine J   Brodsky Frances M FM   Walters Kylie J KJ   Polo Simona S  

Nature communications 20191031 1


Clathrin light chains (CLCa and CLCb) are major constituents of clathrin-coated vesicles. Unique functions for these evolutionary conserved paralogs remain elusive, and their role in clathrin-mediated endocytosis in mammalian cells is debated. Here, we find and structurally characterize a direct and selective interaction between CLCa and the long isoform of the actin motor protein myosin VI, which is expressed exclusively in highly polarized tissues. Using genetically-reconstituted Caco-2 cysts  ...[more]

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