Ontology highlight
ABSTRACT:
SUBMITTER: Palanisamy N
PROVIDER: S-EPMC6823396 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Palanisamy Navaneethan N Degen Anna A Morath Anna A Ballestin Ballestin Jara J Juraske Claudia C Öztürk Mehmet Ali MA Sprenger Georg A GA Youn Jung-Won JW Schamel Wolfgang W WW Di Ventura Barbara B
Nature communications 20191031 1
To build or dissect complex pathways in bacteria and mammalian cells, it is often necessary to recur to at least two plasmids, for instance harboring orthogonal inducible promoters. Here we present SiMPl, a method based on rationally designed split enzymes and intein-mediated protein trans-splicing, allowing the selection of cells carrying two plasmids with a single antibiotic. We show that, compared to the traditional method based on two antibiotics, SiMPl increases the production of the antimi ...[more]