Analysis of thrombin-antithrombin complex formation using microchip electrophoresis and mass spectrometry.
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ABSTRACT:
SUBMITTER: Nielsen JB
PROVIDER: S-EPMC6829041 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Publications
Analysis of thrombin-antithrombin complex formation using microchip electrophoresis and mass spectrometry.
Nielsen Jacob B JB Nielsen Anna V AV Carson Richard H RH Lin Hsien-Jung L HL Hanson Robert L RL Sonker Mukul M Mortensen Daniel N DN Price John C JC Woolley Adam T AT
Electrophoresis 20190813 21
Preterm birth (PTB) related health problems take over one million lives each year, and currently, no clinical analysis is available to determine if a fetus is at risk for PTB. Here, we describe the preparation of a key PTB risk biomarker, thrombin-antithrombin (TAT), and characterize it using dot blots, MS, and microchip electrophoresis (µCE). The pH for fluorescently labeling TAT was also optimized using spectrofluorometry and spectrophotometry. The LOD of TAT was measured in µCE. Lastly, TAT w ...[more]
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