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Genomic characterization of a pectinolytic isolate of Serratia oryzae isolated from lake water.

ABSTRACT: Only one isolate of Serratia oryzae, the type strain J11-6^T has been characterized up to now. This strain was found in the endophytic bacterial flora of rice. As part of an ongoing investigation into pectinolytic bacteria present in lake water in France, a few Serratia strains were isolated, including S32 and J9 identified as new strains of S. oryzae. The genome of strain S32 consists of a circular chromosome of 4,810,389 bp that contains 4,584 protein-coding genes. The genome of S32, as well as those of the type strain J11-6^T, contains several genes involved in pectin degradation and in the intracellular assimilation of pectin oligomers. The specific detection of enzyme activities confirmed that strain S32 secretes functional pectinases and that it also produces extracellular cellulase and protease activities. The ability to produce plant cell wall degrading enzymes shows that S. oryzae shares characteristics of plant associated bacteria, including phytopathogens.

SUBMITTER: Hugouvieux-Cotte-Pattat N

PROVIDER: S-EPMC6831795 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Genomic characterization of a pectinolytic isolate of Serratia oryzae isolated from lake water.

Hugouvieux-Cotte-Pattat Nicole N Jacot-des-Combes Cécile C Briolay Jérôme J

Journal of genomics 20191015

Only one isolate of Serratia oryzae, the type strain J11-6T has been characterized up to now. This strain was found in the endophytic bacterial flora of rice. As part of an ongoing investigation into pectinolytic bacteria present in lake water in France, a few Serratia strains were isolated, including S32 and J9 identified as new strains of S. oryzae. The genome of strain S32 consists of a circular chromosome of 4,810,389 bp that contains 4,584 protein-coding gene ...[more]

PMID: 31719848

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Project description:Strains of Serratia marcescens, originally isolated from the gut lumen of adult female Anopheles stephensi mosquitoes, established persistent infection at high rates in adult A. stephensi whether fed to larvae or in the sugar meal to adults. By contrast, the congener S. fonticola originating from Aedes triseriatus had lower infection in A. stephensi, suggesting co-adaptation of Serratia strains in different species of host mosquitoes. Coinfection at high infection rate in adult A. stephensi resulted after feeding S. marcescens and Elizabethkingia anophelis in the sugar meal, but when fed together to larvae, infection rates with E. anophelis were much higher than were S. marcescens in adult A. stephensi, suggesting a suppression effect of coinfection across life stages. A primary isolate of S. marcescens was resistant to all tested antibiotics, showed high survival in the mosquito gut, and produced alpha-hemolysins which contributed to lysis of erythrocytes ingested with the blood meal. Genomes of two primary isolates from A. stephensi, designated S. marcescens ano1 and ano2, were sequenced and compared to other Serratia symbionts associated with insects, nematodes and plants. Serratia marcescens ano1 and ano2 had predicted virulence factors possibly involved in attacking parasites and/or causing opportunistic infection in mosquito hosts. S. marcescens ano1 and ano2 possessed multiple mechanisms for antagonism against other microorganisms, including production of bacteriocins and multi-antibiotic resistance determinants. These genes contributing to potential anti-malaria activity including serralysins, hemolysins and chitinases are only found in some Serratia species. It is interesting that genome sequences in S. marcescens ano1 and ano2 are distinctly different from those in Serratia sp. Ag1 and Ag2 which were isolated from Anopheles gambiae. Compared to Serratia sp. Ag1 and Ag2, S. marcescens ano1 and ano2 have more rRNAs and many important genes involved in commensal and anti-parasite traits.

Dataset Information

Genomic characterization of a pectinolytic isolate of Serratia oryzae isolated from lake water.

Publications

Genomic characterization of a pectinolytic isolate of <i>Serratia oryzae</i> isolated from lake water.

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