Ontology highlight
ABSTRACT:
SUBMITTER: Heo JM
PROVIDER: S-EPMC6834391 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Heo Jin-Mi JM Harper Nathan J NJ Paulo Joao A JA Li Mamie M Xu Qikai Q Coughlin Margaret M Elledge Stephen J SJ Harper J Wade JW
Science advances 20191106 11
The PINK1 protein kinase activates the PARK2 ubiquitin ligase to promote mitochondrial ubiquitylation and recruitment of ubiquitin-binding mitophagy receptors typified by OPTN and TAX1BP1. Here, we combine proximity biotinylation of OPTN and TAX1BP1 with CRISPR-Cas9-based screens for mitophagic flux to develop a spatial proteogenetic map of PARK2-dependent mitophagy. Proximity labeling of OPTN allowed visualization of a "mitochondrial-autophagosome synapse" upon mitochondrial depolarization. Pro ...[more]