Ontology highlight
ABSTRACT:
SUBMITTER: Eisenstein F
PROVIDER: S-EPMC6839400 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Eisenstein Fabian F Danev Radostin R Pilhofer Martin M
Journal of structural biology 20190816 2
The power of cryo-electron tomography (cryoET) lies in its capability to characterize macromolecules in their cellular context. Structure determination by cryoET, however, is time-consuming compared to single particle approaches. A recent study reported significant acceleration of data acquisition by a fast-incremental single-exposure (FISE) tilt series scheme. Here we improved the method and evaluated its efficiency and performance. We show that (1) FISE combined with the latest generation of d ...[more]