Improved applicability and robustness of fast cryo-electron tomography data acquisition
Ontology highlight
ABSTRACT:
SUBMITTER: Martin Pilhofer
PROVIDER: EMPIAR-10304 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Improved applicability and robustness of fast cryo-electron tomography data acquisition.
Eisenstein Fabian F Danev Radostin R Pilhofer Martin M
Journal of structural biology 20190816 2
The power of cryo-electron tomography (cryoET) lies in its capability to characterize macromolecules in their cellular context. Structure determination by cryoET, however, is time-consuming compared to single particle approaches. A recent study reported significant acceleration of data acquisition by a fast-incremental single-exposure (FISE) tilt series scheme. Here we improved the method and evaluated its efficiency and performance. We show that (1) FISE combined with the latest generation of d ...[more]
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