Ontology highlight
ABSTRACT:
SUBMITTER: Pandey D
PROVIDER: S-EPMC6842382 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
Pandey Deepak D Patel Sanjay K S SKS Singh Rajendra R Kumar Pradeep P Thakur Vikram V Chand Duni D
Indian journal of microbiology 20191104 4
Amidase from <i>Bacillus</i> sp. APB-6 with very good acyltransferase activity was purified to homogeneity with a purification fold of 3.68 and 53.20% enzyme yield. The purified protein's subunit molecular mass was determined approximately 42 kDa. Hyperactivity of the enzyme was observed at pH 7.5 (150 mM, potassium-phosphate buffer) and 50 °C of incubation. An enhancement in activity up to 42% was recorded with ethylenediaminetetraacetic acid and dithiothreitol. The kinetic parameter <i>K</i> < ...[more]