MoCloFlex: A Modular Yet Flexible Cloning System.
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ABSTRACT:
SUBMITTER: Klein CA
PROVIDER: S-EPMC6843054 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Publications
MoCloFlex: A Modular Yet Flexible Cloning System.
Klein Carlo A CA Emde Leonie L Kuijpers Aaron A Sobetzko Patrick P
Frontiers in bioengineering and biotechnology 20191017
Modern cloning solutions are gradually replacing classical cloning methods. Current systems make use of libraries with predefined DNA parts that are joined by Golden-Gate reactions. However, these systems still suffer from specific inflexibilities and the lack of inter-compatibility. Here, we present Flexible Modular Cloning (MoCloFlex) which overcomes this inflexibility by introducing a set of linker- and position-vectors allowing free unit arrangement. Our system, therefore, provides a conveni ...[more]
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