Ontology highlight
ABSTRACT:
SUBMITTER: Fu Q
PROVIDER: S-EPMC6845026 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Fu Qin Q Kowalski Michael P MP Mastali Mitra M Parker Sarah J SJ Sobhani Kimia K van den Broek Irene I Hunter Christie L CL Van Eyk Jennifer E JE
Journal of proteome research 20171110 1
Sample preparation for protein quantification by mass spectrometry requires multiple processing steps including denaturation, reduction, alkylation, protease digestion, and peptide cleanup. Scaling these procedures for the analysis of numerous complex biological samples can be tedious and time-consuming, as there are many liquid transfer steps and timed reactions where technical variations can be introduced and propagated. We established an automated sample preparation workflow with a total proc ...[more]