Ontology highlight
ABSTRACT:
SUBMITTER: Klont F
PROVIDER: S-EPMC5906755 | biostudies-literature | 2018 Apr
REPOSITORIES: biostudies-literature
Klont Frank F Bras Linda L Wolters Justina C JC Ongay Sara S Bischoff Rainer R Halmos Gyorgy B GB Horvatovich Péter P
Analytical chemistry 20180406 8
For mass spectrometry-based proteomics, the selected sample preparation strategy is a key determinant for information that will be obtained. However, the corresponding selection is often not based on a fit-for-purpose evaluation. Here we report a comparison of in-gel (IGD), in-solution (ISD), on-filter (OFD), and on-pellet digestion (OPD) workflows on the basis of targeted (QconCAT-multiple reaction monitoring (MRM) method for mitochondrial proteins) and discovery proteomics (data-dependent acqu ...[more]