Ontology highlight
ABSTRACT:
SUBMITTER: Beier DH
PROVIDER: S-EPMC6846040 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Beier David H DH Carrocci Tucker J TJ van der Feltz Clarisse C Tretbar U Sandy US Paulson Joshua C JC Grabowski Nikolai N Hoskins Aaron A AA
Nucleic acids research 20191101 20
The DEAD-box family of proteins are ATP-dependent, RNA-binding proteins implicated in many aspects of RNA metabolism. Pre-mRNA splicing in eukaryotes requires three DEAD-box ATPases (Prp5, Prp28 and Sub2), the molecular mechanisms of which are poorly understood. Here, we use single molecule FRET (smFRET) to study the conformational dynamics of yeast Prp5. Prp5 is essential for stable association of the U2 snRNP with the intron branch site (BS) sequence during spliceosome assembly. Our data show ...[more]