Label-free multiphoton imaging of ?-amyloid plaques in Alzheimer's disease mouse models.

ABSTRACT: ? -Amyloid ( A ? ) plaque, representing the progressive accumulation of the protein that mainly consists of A ? , is one of the prominent pathological hallmarks of Alzheimer's disease (AD). Label-free imaging of A ? plaques holds the potential to be a histological examination tool for diagnosing AD. We applied label-free multiphoton microscopy to identify extracellular A ? plaque as well as intracellular A ? accumulation for the first time from AD mouse models. We showed that a two-photon-excited fluorescence signal is a sensitive optical marker for revealing the spatial-temporal progression and the surrounding morphological changes of A ? deposition, which demonstrated that both extracellular and intracellular A ? accumulations play an important role in the progression of AD. Moreover, combined with a custom-developed image-processing program, we established a rapid method to visualize different degrees of A ? deposition by color coding. These results provide an approach for investigating pathophysiology of AD that can complement traditional biomedical procedures.

SUBMITTER: Wang S 

PROVIDER: S-EPMC6850002 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature