Dataset Information
Impact of cold storage on platelets treated with Intercept pathogen inactivation.
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ABSTRACT: Background
Pathogen inactivation and cold or cryopreservation of platelets (PLTs) both significantly affect PLT function. It is not known how PLTs function when both are combined.Study design and methods
Standard PLT concentrates (PCs) were compared to pathogen-inactivated PCs treated with amotosalen photochemical treatment (AS-PCT) when stored at room (RT, 22°C), cold (4°C, n = 6), or cryopreservation (-80°C, n = 8) temperatures. The impact of alternative storage methods on both arms was studied in flow cytometry, light transmittance aggregometry, and hemostasis in collagen-coated microfluidic flow chambers.Results
Platelet aggregation of cold-stored AS-PCT PLTs was 44%?±?11% compared to 57%?±?14% for cold-stored standard PLTs and 58%?±?21% for RT-stored AS-PCT PLTs. Integrin activation of cold-stored AS-PCT PLTs was 53%?±?9% compared to 77%?±?6% for cold-stored standard PLTs and 69%?±?13% for RT-stored AS-PCT PLTs. Coagulation of cold-stored AS-PCT PLTs started faster under flow (836?±?140?sec) compared to cold-stored standard PLTs (960?±?192?sec) and RT-stored AS-PCT PLTs (1134?±?220?sec). Fibrin formation rate under flow was also highest for cold-stored AS-PCT PLTs. This was in line with thrombin generation in static conditions because cold-stored AS-PCT PLTs generated 297?±?47?nmol/L thrombin compared to 159?±?33?nmol/L for cold-stored standard PLTs and 83?±?25?nmol/L for RT-stored AS-PCT PLTs. So despite decreased PLT activation and aggregation, cold storage of AS-PCT PLTs promoted coagulation. PLT aggregation of cryopreserved AS-PCT PLTs (23%?±?10%) was not significantly different from cryopreserved standard PLTs (25%?±?8%).Conclusion
This study shows that cold storage of AS-PCT PLTs further affects PLT activation and aggregation but promotes (pro)coagulation. Increased procoagulation was not observed after cryopreservation.
SUBMITTER: Six KR
PROVIDER: S-EPMC6851707 | biostudies-literature | 2019 Aug
REPOSITORIES: biostudies-literature
Publications
Impact of cold storage on platelets treated with Intercept pathogen inactivation.
Transfusion 20190612 8
<h4>Background</h4>Pathogen inactivation and cold or cryopreservation of platelets (PLTs) both significantly affect PLT function. It is not known how PLTs function when both are combined.<h4>Study design and methods</h4>Standard PLT concentrates (PCs) were compared to pathogen-inactivated PCs treated with amotosalen photochemical treatment (AS-PCT) when stored at room (RT, 22°C), cold (4°C, n = 6), or cryopreservation (-80°C, n = 8) temperatures. The impact of alternative storage methods on both ...[more]
