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Compartments for Synthetic Cells: Osmotically Assisted Separation of Oil from Double Emulsions in a Microfluidic Chip.

ABSTRACT: Liposomes are used in synthetic biology as cell-like compartments and their microfluidic production through double emulsions allows for efficient encapsulation of various components. However, residual oil in the membrane remains a critical bottleneck for creating pristine phospholipid bilayers. It has been discovered that osmotically driven shrinking leads to detachment of the oil drop. Separation inside a microfluidic chip has been realized to automate the procedure, which allows for controlled continuous production of monodisperse liposomes.

SUBMITTER: Krafft D

PROVIDER: S-EPMC6852271 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Compartments for Synthetic Cells: Osmotically Assisted Separation of Oil from Double Emulsions in a Microfluidic Chip.

Krafft Dorothee D López Castellanos Sebastián S Lira Rafael B RB Dimova Rumiana R Ivanov Ivan I Sundmacher Kai K

Chembiochem : a European journal of chemical biology 20190730 20

Liposomes are used in synthetic biology as cell-like compartments and their microfluidic production through double emulsions allows for efficient encapsulation of various components. However, residual oil in the membrane remains a critical bottleneck for creating pristine phospholipid bilayers. It has been discovered that osmotically driven shrinking leads to detachment of the oil drop. Separation inside a microfluidic chip has been realized to automate the procedure, which allows for controlled ...[more]

PMID: 31090995

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