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Metagenomic data of the microbial community of lab-scale nitritation-anammox sequencing-batch bioreactor performing nitrogen removal from synthetic wastewater.

ABSTRACT: The nitritation-anammox process, which involves partial aerobic oxidation of the ammonium to nitrite and following oxidation of ammonium by nitrite to molecular nitrogen, is an efficient and cost-effective approach for biological nitrogen removal from wastewater. To characterize the microbial communities involved in the nitrogen and carbon cycles in wastewater treatment bioreactors employing this process, we sequenced the metagenome of a sludge sample collected from the lab-scale nitritation-anammox sequencing-batch reactor. At the phylum level, Proteobacteria and Chloroflexi were the most numerous groups. Anammox bacteria belonged to the genus Candidatus Brocadia. The obtained data will help to investigate the taxonomical and functional diversity the microbial communities involved in nitritation-anammox process, and will be used for genome-based analysis of uncultured bacterial lineages. The raw sequencing data is available from the NCBI Sequence Read Archive (SRR9831403) database under the BioProject PRJN0A55627.

SUBMITTER: Mardanov AV

PROVIDER: S-EPMC6859213 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Metagenomic data of the microbial community of lab-scale nitritation-anammox sequencing-batch bioreactor performing nitrogen removal from synthetic wastewater.

Mardanov Andrey V AV Kotlyarov Roman V RV Beletsky Alexey V AV Nikolaev Yury A YA Kallistova Anna Yu AY Grachev Vladimir A VA Berestovskaya Yulia Yu YY Pimenov Nikolai V NV Ravin Nikolai V NV

Data in brief 20191104

The nitritation-anammox process, which involves partial aerobic oxidation of the ammonium to nitrite and following oxidation of ammonium by nitrite to molecular nitrogen, is an efficient and cost-effective approach for biological nitrogen removal from wastewater. To characterize the microbial communities involved in the nitrogen and carbon cycles in wastewater treatment bioreactors employing this process, we sequenced the metagenome of a sludge sample collected from the lab-scale nitritation-ana ...[more]

PMID: 31763390

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Project description:Anaerobic ammonia oxidizing bacteria (Anammox) are implemented in high-efficiency wastewater treatment systems operated in two general configurations; one-stage systems combine aerobic ammonia oxidizing bacteria (AOB) and Anammox within a single aerated reactor, whereas two-stage configurations separate these processes into discrete tanks. Within both configurations heterotrophic populations that perform denitrification or dissimilatory nitrate reduction to ammonia (DNRA) compete for carbon and nitrate or nitrite and can impact reactor performance because DNRA retains nitrogen in the system. Therefore, it is important to understand how selective pressures imposed by one-stage and two-stage reactor configurations impact the microbial community structure and associated nitrogen transforming functions. We performed 16S rRNA gene and metagenomic sequencing on different biomass fractions (granules, flocs, and suspended biomass) sampled from two facilities treating sludge dewatering centrate: a one-stage treatment facility (Chambers Creek, Tacoma, WA) and a two-stage system (Rotterdam, Netherlands). Similar microbial populations were identified across the different samples, but relative abundances differed between reactor configurations and biomass sources. Analysis of metagenome assembled genomes (MAGs) indicated different lifestyles for abundant heterotrophic populations. Acidobacteria, Bacteroidetes, and Chloroflexi MAGs had varying capacity for DNRA and denitrification. Acidobacteria MAGs possessed high numbers of glycosyl hydrolases and glycosyl transferases indicating a role in biomass degradation. Ignavibacteria and Phycosphaerae MAGs contributed to the greater relative abundance of DNRA associated nrf genes in the two-stage granules and contained genomic features suggesting a preference for an anoxic or microoxic niche. In the one-stage granules a MAG assigned to Burkholderiales accounted for much of the abundant denitrification genes and had genomic features, including the potential for autotrophic denitrification using reduced sulfur, that indicate an ability to adapt its physiology to varying redox conditions. Overall, the competition for carbon substrates between denitrifying and DNRA performing heterotrophs may be impacted by configuration specific selective pressures. In one-stage systems oxygen availability in the bulk liquid and the oxygen gradient within granules would provide a greater niche space for heterotrophic populations capable of utilizing both oxygen and nitrate or nitrite as terminal electron acceptors, compared to two-stage systems where a homogeneous anoxic environment would favor heterotrophic populations primarily adapted to anaerobic metabolism.

Project description:This study investigated the potential of Membrane-Aerated Biofilm Reactors (MABRs) for mainstream nitrogen removal via partial nitration/anaerobic ammonium oxidation (anammox). Four laboratory-scale MABRs were operated with real municipal wastewater characterized by low concentrations of nitrogen (varying between 31 and 120 mg-NH4-N L-1) and the presence of biodegradable organic carbon (soluble COD (sCOD) between 7 and 230 mg-O2 L-1). Two reactors were operated with different aeration strategies (intermittent vs. continuous), the other two with differences in biomass retention (recirculation or removal of detached biomass). Keeping a constant HRT caused instabilities due to difficulties with setting the optimal oxygen flux for the respective surface loadings (1.6-6 g-NH4-N m-2 d-1). Operating the MABRs with a constant surface loading (2 g-NH4-N m-2 d-1) resulted in higher and more stable total nitrogen (TN) removal independent of the aeration strategy. The intermittently aerated MABR improved from an average TN removal of 23%-69%, the continuously aerated MABR from 20% to 50% TN removal. Independent of the feeding strategy, the continuously aerated reactor removed slightly more ammonium (80-95%) compared to the intermittently aerated reactor (74-93%). Limiting the oxygen supply by intermittent aeration proofed successful to favor partial nitritation and anammox. Continuous aeration did not achieve stable suppression of nitrite oxidizing bacteria (NOB). Of the removed ammonium, approx. 26% were left in the effluent as nitrate (only 10% with intermittent aeration). Recirculation of the detached biomass resulted in reattachment onto the biofilm or membrane surface. This recirculation led to significantly higher biomass retention times and thus to better performance. Removing detached biofilm from the reactor caused a slightly lower TN removal of 33% compared to 45% with reattachment, while average ammonium removal was 58% compared to 63%, respectively. Scouring events had a significant impact on the overall operation, resulting in short term losses of TN removal capacities of 50-100%. The microbial community composition was different depending on the aeration strategy and biomass retention. The continuously aerated reactor contained significantly more AOB than the intermittently aerated MABR. The reactor with biomass retention contained less ammonium oxidizing bacteria (AOB), compared to the reactor with low biomass retention. In all MABRs, anammox bacteria established in the biofilm after an initial drop in abundance.

Project description:The partial nitritation-anaerobic ammonium oxidation (anammox; PN-A) process has been considered a sustainable method for wastewater ammonium removal, with recent attempts to treat low-strength wastewater. However, how microbes adapt to the alternate microaerobic-anoxic operation of the process when treating low ammonium concentrations remains poorly understood. In this study, we applied a metagenomic approach to determine the genomic contents of core members in a PN-A reactor treating inorganic ammonium wastewater at loading as low as 0.0192 kg-N/m3/day. The metabolic traits of metagenome-assembled genomes from 18 core species were analyzed. Taxonomically diverse ammonia oxidizers, including two Nitrosomonas species, a comammox Nitrospira species, a novel Chloroflexota-related species, and two anammox bacteria, Ca. Brocadia and Ca. Jettenia, accounted for the PN-A reactions. The characteristics of a series of genes encoding class II ribonucleotide reductase, high-affinity bd-type terminal oxidase, and diverse antioxidant enzymes revealed that comammox Nitrospira has a superior adaptation ability over the competitors, which may confer the privileged partnership with anammox bacteria in the PN-A reactor. This finding is supported by the long-term monitoring experiment, showing the predominance of the comammox Nitrospira in the ammonia-oxidizing community. Metagenomic analysis of seven heterotrophs suggested that nitrate reduction is a common capability in potentially using endogenous carbohydrates and peptides to enhance nitrogen removals. The prevalence of class II ribonucleotide reductase and antioxidant enzymes genes may grant the adaptation to cyclically microaerobic/anoxic environments. The predominant heterotroph is affiliated with Chloroflexota; its genome encodes complete pathways for synthesizing vitamin B6 and methionine. By contrast, other than the two growth factors, Nitrospira and anammox bacteria are complementary to produce various vitamins and amino acids. Besides, the novel Chloroflexota-related ammonia oxidizer lacks corresponding genes for detoxifying the reactive oxygen species and thus requires the aid of co-existing members to alleviate oxidative stress. The analysis results forecast the exchanges of substrates and nutrients as well as the collective alleviation of oxidative stress among the core populations. The new findings of the genomic features and predicted microbial interplay shed light on microbial adaptation to intermittent microaeration specific to the PN-A reactor, which may aid in improving its application to low-strength ammonium wastewater.

Dataset Information

Metagenomic data of the microbial community of lab-scale nitritation-anammox sequencing-batch bioreactor performing nitrogen removal from synthetic wastewater.

Publications

Metagenomic data of the microbial community of lab-scale nitritation-anammox sequencing-batch bioreactor performing nitrogen removal from synthetic wastewater.

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