Dataset Information
Diagnostic plasma miRNA-profiles for ovarian cancer in patients with pelvic mass.
Ontology highlight
ABSTRACT: Background
Ovarian cancer is the fifth most common cancer in women worldwide. Moreover, there are no reliable minimal invasive tests to secure the diagnosis of malignant pelvic masses. Cell-free, circulating microRNAs have the potential as diagnostic biomarkers in cancer. Here, we performed and validated a miRNA panel with the potential to distinguish OC from benign pelvic masses.Methods
The profile of plasma microRNA was determined with a panel of 46 candidates in a discovery group and a validation group, each consisting of 190 pre-surgery plasma samples from age-matched patients with malignant (n = 95) and benign pelvic mass (n = 95), by real time RT-qPCR.Results
Four up-regulated (miR-200c-3p, miR-221-3p, miR-21-5p, and miR-484) and two down-regulated (miR-195-5p and miR-451a) microRNAs were discovered. From those, miR-200c-3p and miR-221-3p were further confirmed in a validation cohort. A combination of these 2 microRNAs together with CA-125 yielded an overall diagnostic accuracy of AUC = 0.96.Conclusions
We showed consistent plasma microRNA profiles that provide independent diagnostic information of late stage OC.
SUBMITTER: Oliveira DNP
PROVIDER: S-EPMC6860451 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Publications
Diagnostic plasma miRNA-profiles for ovarian cancer in patients with pelvic mass.
PloS one 20191118 11
<h4>Background</h4>Ovarian cancer is the fifth most common cancer in women worldwide. Moreover, there are no reliable minimal invasive tests to secure the diagnosis of malignant pelvic masses. Cell-free, circulating microRNAs have the potential as diagnostic biomarkers in cancer. Here, we performed and validated a miRNA panel with the potential to distinguish OC from benign pelvic masses.<h4>Methods</h4>The profile of plasma microRNA was determined with a panel of 46 candidates in a discovery gr ...[more]
