Dataset Information

Plasma miRNA profiles associated with stable warfarin dosage in Chinese patients.

ABSTRACT: Background:We used bioinformatic analysis and quantitative reverse transcription polymerase chain reaction (RT-qPCR) assays to investigate the association between plasma microRNAs (miRNAs) and stable warfarin dosage in a Chinese Han population. Methods:Bioinformatics analysis was used to screen out potential warfarin dose-associated miRNAs. Three plasma miRNAs were validated in 99 samples by RT-qPCR. Kruskal-Wallis test and multivariate logistic regression were used to compare differences in plasma miRNAs expression levels between three warfarin dosage groups. Results:There were significant between-group differences among the three dose groups for hsa-miR-133b expression (p = 0.005), but we observed an "n-shaped" dose-dependent curve rather than a linear relationship. Expression levels of hsa-miR-24-3p (p = 0.475) and hsa-miR-1276 (p = 0.558) were not significantly different in the multivariate logistic regression. Conclusion:miRNAs have received extensive attention as ideal biomarkers and possible therapeutic targets for various diseases. However, they are not yet widely used in precision medicine. Our results indicate that hsa-miR-133b may be a possible reference factor for the warfarin dosage algorithm. These findings emphasize the importance of a comprehensive evaluation of complex relationships in warfarin dose prediction models and provide new avenues for future pharmacogenomics studies.

SUBMITTER: Zhao L

PROVIDER: S-EPMC7566751 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Plasma miRNA profiles associated with stable warfarin dosage in Chinese patients.

Zhao Li L Wang Jin J Shi Shaoxin S Wu Yuan Y Liu Jumei J He Shiwei S Zou Yue Y Xie Huabin H Ge Shengxiang S Ye Huiming H

PeerJ 20201013

<h4>Background</h4>We used bioinformatic analysis and quantitative reverse transcription polymerase chain reaction (RT-qPCR) assays to investigate the association between plasma microRNAs (miRNAs) and stable warfarin dosage in a Chinese Han population.<h4>Methods</h4>Bioinformatics analysis was used to screen out potential warfarin dose-associated miRNAs. Three plasma miRNAs were validated in 99 samples by RT-qPCR. Kruskal-Wallis test and multivariate logistic regression were used to compare dif ...[more]

PMID: 33083118

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Dataset Information

Plasma miRNA profiles associated with stable warfarin dosage in Chinese patients.

Publications

Plasma miRNA profiles associated with stable warfarin dosage in Chinese patients.

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