Measurement of murine kidney functional biomarkers using DCE-MRI: A multi-slice TRICKS technique and semi-automated image processing algorithm.

ABSTRACT: PURPOSE:To propose a rapid multi-slice T1 measurement method using time-resolved imaging of contrast kinetics (TRICKS) and a semi-automated image processing algorithm for comprehensive assessment murine kidney function using dynamic contrast-enhanced MRI (DCE-MRI). METHODS:A multi-slice TRICKS sampling scheme was implemented in an established rapid T1 measurement method. A semi-automated image-processing scheme employing basic image processing techniques and machine learning was developed to facilitate image analysis. Reliability of the multi-slice technique in measuring renal perfusion and glomerular filtration rate (GFR) was tested in normal mice (n?=?7 for both techniques) by comparing to the validated single-slice technique. Utility of this method was demonstrated on mice after either sham surgery (n?=?7) or induction of unilateral renal artery stenosis (RAS, n?=?8). Renal functional parameters were extracted using a validated bi-compartment model. RESULTS:The TRICKS sampling scheme achieved an acceleration factor of 2.7, allowing imaging of eight axial slices at 1.23?s/scan. With the aid of the semi-automated scheme, image analysis required under 15-min for both kidneys per mouse. The multi-slice technique yielded renal perfusion and GFR values comparable to the single-slice technique. Model-fitted renal parameters successfully differentiated control and stenotic mouse kidneys, including renal perfusion (706.5?±?164.0 vs. 375.9?±?277.9?mL/100?g/min, P?=?0.002), blood flow (1.6?±?0.4 vs. 0.7?±?0.7?mL/min, P?

SUBMITTER: Jiang K 

PROVIDER: S-EPMC6861697 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature