Dataset Information

Global change-driven use of onshore habitat impacts polar bear faecal microbiota.

ABSTRACT: The gut microbiota plays a critical role in host health, yet remains poorly studied in wild species. Polar bears (Ursus maritimus), key indicators of Arctic ecosystem health and environmental change, are currently affected by rapid shifts in habitat that may alter gut homeostasis. Declining sea ice has led to a divide in the southern Beaufort Sea polar bear subpopulation such that an increasing proportion of individuals now inhabit onshore coastal regions during the open-water period ('onshore bears') while others continue to exhibit their typical behaviour of remaining on the ice ('offshore bears'). We propose that bears that have altered their habitat selection in response to climate change will exhibit a distinct gut microbiota diversity and composition, which may ultimately have important consequences for their health. Here, we perform the first assessment of abundance and diversity in the faecal microbiota of wild polar bears using 16S rRNA Illumina technology. We find that bacterial diversity is significantly higher in onshore bears compared to offshore bears. The most enriched OTU abundance in onshore bears belonged to the phylum Proteobacteria, while the most depleted OTU abundance within onshore bears was seen in the phylum Firmicutes. We conclude that climate-driven changes in polar bear land use are associated with distinct microbial communities. In doing so, we present the first case of global change mediated alterations in the gut microbiota of a free-roaming wild animal.

SUBMITTER: Watson SE

PROVIDER: S-EPMC6864082 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Global change-driven use of onshore habitat impacts polar bear faecal microbiota.

Watson Sophie E SE Hauffe Heidi C HC Bull Matthew J MJ Atwood Todd C TC McKinney Melissa A MA Pindo Massimo M Perkins Sarah E SE

The ISME journal 20190805 12

The gut microbiota plays a critical role in host health, yet remains poorly studied in wild species. Polar bears (Ursus maritimus), key indicators of Arctic ecosystem health and environmental change, are currently affected by rapid shifts in habitat that may alter gut homeostasis. Declining sea ice has led to a divide in the southern Beaufort Sea polar bear subpopulation such that an increasing proportion of individuals now inhabit onshore coastal regions during the open-water period ('onshore b ...[more]

PMID: 31378786

Similar Datasets

Project description:Greenhouse-gas-induced warming in the Arctic has caused declines in sea ice extent and changed its composition, raising concerns by all circumpolar nations for polar bear conservation.Negative impacts have been observed in three well-studied polar bear subpopulations. Most subpopulations, however, receive little or no direct monitoring, hence, resource selection functions (RSF) may provide a useful proxy of polar bear distributions. However, the efficacy of RSFs constructed from past data, that is, reference RSFs, may be degraded under contemporary conditions, especially in a rapidly changing environment.We assessed published Arctic-wide reference RSFs using tracking data from adult female polar bears captured in the Beaufort Sea. We compared telemetry-derived seasonal distributions of polar bears to RSF-defined optimal sea ice habitat during the period of RSF model development, 1985-1995, and two subsequent periods with diminished sea ice: 1996-2006 and 2007-2016. From these comparisons, we assessed the applicability of the reference RSFs for contemporary polar bear conservation.In the two decades following the 1985-1995 reference period, use and availability of optimal habitat by polar bears declined during the ice melt, ice minimum, and ice growth seasons. During the ice maximum season (i.e., winter), polar bears used the best habitat available, which changed relatively little across the three decades of study. During the ice melt, ice minimum, and ice growth seasons, optimal habitat in areas used by polar bears decreased and was displaced north and east of the Alaska Beaufort Sea coast. As optimal habitat diminished in these seasons, polar bears expanded their range and occupied greater areas of suboptimal habitat.Synthesis and applications: Sea ice declines due to climate change continue to challenge polar bears and their conservation. The distribution of Southern Beaufort Sea polar bears remained similar during the ice maximum season, so the reference RSFs developed from data collected >20 years ago continue to accurately model their winter distribution. In contrast, reference RSFs for the ice transitional and minimum seasons showed diminished predictive efficacy but were useful in revealing that contemporary polar bears have been increasingly forced to use suboptimal habitats during those seasons.

Project description:Polar bears in captivity can be exposed to opportunistic pathogens not present in their natural environments. A 4-month-old polar bear (Ursus maritimus) living in an isolated enclosure with his mother in the Tierpark Berlin, Berlin, Germany, was suffering from severe abdominal pain, mild diarrhea, and loss of appetite and died in early 2017. Histopathology revealed severe hepatic degeneration and necrosis without evidence of inflammation or inclusion bodies, although a viral infection had been suspected on the basis of the clinical signs. We searched for nucleic acids of pathogens by shotgun high-throughput sequencing (HTS) from genomic DNA and cDNA extracted from tissue and blood. We identified a novel Mastadenovirus and assembled a nearly complete genome from the shotgun sequences. Quantitative PCR (qPCR) revealed that viral DNA was present in various concentrations in all tissues examined and that the highest concentrations were found in blood. Viral culture did not yield cytopathic effects, but qPCR suggested that virus replication was sustained for up to three passages. Positive immunofluorescence staining confirmed that the virus was able to replicate in the cells during early passage. Phylogenetic analysis demonstrated that the virus is highly divergent compared to other previously identified Mastadenovirus members and basal to most known viral clades. The virus was found only in the 4-month-old bear and not in other captive polar bears tested. We surmised, therefore, that the polar bear was infected from an unknown reservoir, illustrating that adenoviral diversity remains underestimated and that cross-species transmission of viruses can occur even under conditions of relative isolation.IMPORTANCE Cross-species transmission of viral pathogens is becoming an increasing problem for captive-animal facilities. This study highlights how animals in captivity are vulnerable to novel opportunistic pathogens, many of which do not result in straightforward diagnosis from symptoms and histopathology. In this study, a novel pathogen was suspected to have contributed to the death of a juvenile polar bear. HTS techniques were employed, and a novel Mastadenovirus was isolated. The virus was present in both the tissue and blood samples. Phylogenetic analysis of the virus at both the gene and genome levels revealed that it is highly divergent to other known mastadenoviruses. Overall, this study shows that animals in isolated conditions still come into contact with novel pathogens, and for many of these pathogens, the host reservoir and mode of transmission are yet to be determined.

Dataset Information

Global change-driven use of onshore habitat impacts polar bear faecal microbiota.

Publications

Global change-driven use of onshore habitat impacts polar bear faecal microbiota.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets