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Differential distributions of trafficking and signaling proteins of the maize ER-Golgi apparatus.

ABSTRACT: The Endoplasmic Reticulum (ER)-Golgi apparatus of plants is the site of synthesis of non-cellulosic polysaccharides that then traffic to the cell wall. A two-step protocol of flotation centrifugation followed by free-flow electrophoresis (FFE) resolved ER and Golgi proteins into three profiles: an ER-rich fraction, two Golgi-rich fractions, and an intermediate fraction enriched in cellulose synthases. Nearly three dozen Rab-like proteins of eight different subgroups were distributed differentially in ER- vs. Golgi-rich fractions, whereas seven 14-3-3 proteins co-fractionated with cellulose synthases in the intermediate fraction. FFE offers a powerful means to classify resident and transient proteins in cell-free assays of cellular location.

SUBMITTER: Okekeogbu IO

PROVIDER: S-EPMC6866702 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Differential distributions of trafficking and signaling proteins of the maize ER-Golgi apparatus.

Okekeogbu Ikenna O IO Aryal Uma K UK Fernández-Niño Susana M González SMG Penning Bryan W BW Heazlewood Joshua L JL McCann Maureen C MC Carpita Nicholas C NC

Plant signaling & behavior 20190928 12

The Endoplasmic Reticulum (ER)-Golgi apparatus of plants is the site of synthesis of non-cellulosic polysaccharides that then traffic to the cell wall. A two-step protocol of flotation centrifugation followed by free-flow electrophoresis (FFE) resolved ER and Golgi proteins into three profiles: an ER-rich fraction, two Golgi-rich fractions, and an intermediate fraction enriched in cellulose synthases. Nearly three dozen Rab-like proteins of eight different subgroups were distributed differential ...[more]

PMID: 31564200

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