TDCIPP exposure affects Artemia franciscana growth and osmoregulation.

ABSTRACT: Environmental monitoring has demonstrated widespread occurrence of the flame-retardant tris(1,3-dichloro-2-propyl) phosphate (TDCIPP), raising concerns about the impact on aquatic life. Using ¹H NMR and GC-MS metabolomics and 20-day body length experiments, we have determined that exposure to TDCIPP affects Artemia franciscana. The LC₅₀ for a 48?h TDCIPP exposure was determined to be 37.1?±?1.3??M. Acute exposure (48?h) to 20.0??M did not affect A. franciscana body length but did elicit a metabolic change. Chronic exposure to 0.50??M TDCIPP caused decreased body length in A. franciscana exposed for 20?days and elicited a metabolic response. Principal component analysis revealed variance between acute and chronic exposure along PC1 (36.4%) and between control and TDCIPP along PC2 (17.4%). One-way ANOVA indicated that 19 metabolites were significantly affected by TDCIPP exposure; namely metabolites of the osmolyte class, including betaine, phosphocholine, gadusol, taurine, glycerol and trehalose - metabolites that are essential osmoprotectants in extremophile species. Other pathways that may be perturbed by TDCIPP exposure include one carbon, glycine, serine, threonine, and glycerophospholipid metabolism.

SUBMITTER: Morgan MA 

PROVIDER: S-EPMC6868324 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature