Ontology highlight
ABSTRACT:
SUBMITTER: Plangger R
PROVIDER: S-EPMC6868427 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
Plangger Raphael R Juen Michael Andreas MA Hoernes Thomas Philipp TP Nußbaumer Felix F Kremser Johannes J Strebitzer Elisabeth E Klingler David D Erharter Kevin K Tollinger Martin M Erlacher Matthias David MD Kreutz Christoph C
Nucleic acids research 20191201 21
Although group II intron ribozymes are intensively studied the question how structural dynamics affects splicing catalysis has remained elusive. We report for the first time that the group II intron domain 6 exists in a secondary structure equilibrium between a single- and a two-nucleotide bulge conformation, which is directly linked to a switch between sugar puckers of the branch site adenosine. Our study determined a functional sugar pucker equilibrium between the transesterification active C2 ...[more]