Ontology highlight
ABSTRACT:
SUBMITTER: Xu W
PROVIDER: S-EPMC6873407 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Xu Wen W Song Wei W Yang Yongxing Y Wu Ying Y Lv Xinxin X Yuan Shuang S Liu Ya Y Yang Jinxiao J
BMC plant biology 20191121 1
<h4>Background</h4>Application of the CRISPR/Cas9 system or its derived base editors enables targeted genome modification, thereby providing a programmable tool to exploit gene functions and to improve crop traits.<h4>Results</h4>We report that PmCDA1 is much more efficient than rAPOBEC1 when fused to CRISPR/Cas9 nickase for the conversion of cytosine (C) to thymine (T) in rice. Three high-fidelity SpCas9 variants, eSpCas9(1.1), SpCas9-HF2 and HypaCas9, were engineered to serve with PmCDA1 (pBEs ...[more]