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An online resource for GPCR structure determination and analysis.

ABSTRACT: G-protein-coupled receptors (GPCRs) transduce physiological and sensory stimuli into appropriate cellular responses and mediate the actions of one-third of drugs. GPCR structural studies have revealed the general bases of receptor activation, signaling, drug action and allosteric modulation, but so far cover only 13% of nonolfactory receptors. We broadly surveyed the receptor modifications/engineering and methods used to produce all available GPCR crystal and cryo-electron microscopy (cryo-EM) structures, and present an interactive resource integrated in GPCRdb ( http://www.gpcrdb.org ) to assist users in designing constructs and browsing appropriate experimental conditions for structure studies.

SUBMITTER: Munk C

PROVIDER: S-EPMC6881186 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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An online resource for GPCR structure determination and analysis.

Munk Christian C Mutt Eshita E Isberg Vignir V Nikolajsen Louise F LF Bibbe Janne M JM Flock Tilman T Hanson Michael A MA Stevens Raymond C RC Deupi Xavier X Gloriam David E DE

Nature methods 20190121 2

G-protein-coupled receptors (GPCRs) transduce physiological and sensory stimuli into appropriate cellular responses and mediate the actions of one-third of drugs. GPCR structural studies have revealed the general bases of receptor activation, signaling, drug action and allosteric modulation, but so far cover only 13% of nonolfactory receptors. We broadly surveyed the receptor modifications/engineering and methods used to produce all available GPCR crystal and cryo-electron microscopy (cryo-EM) s ...[more]

PMID: 30664776

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