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Real-time monitoring of newly acidified organelles during autophagy enabled by reaction-based BODIPY dyes.


ABSTRACT: Real-time monitoring of newly acidified organelles during autophagy in living cells is highly desirable for a better understanding of intracellular degradative processes. Herein, we describe a reaction-based boron dipyrromethene (BODIPY) dye containing strongly electron-withdrawing diethyl 2-cyanoacrylate groups at the ?-positions. The probe exhibits intense red fluorescence in acidic organelles or the acidified cytosol while exhibiting negligible fluorescence in other regions of the cell. The underlying mechanism is a nucleophilic reaction at the central meso-carbon of the indacene core, resulting in the loss of ?-conjugation entailed by dramatic spectroscopic changes of more than 200?nm between its colorless, non-fluorescent leuco-BODIPY form and its red and brightly emitting form. The reversible transformation between red fluorescent BODIPY and leuco-BODIPY along with negligible cytotoxicity qualifies such dyes for rapid and direct intracellular lysosome imaging and cytosolic acidosis detection simultaneously without any washing step, enabling the real-time monitoring of newly acidified organelles during autophagy.

SUBMITTER: Liu H 

PROVIDER: S-EPMC6883057 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Real-time monitoring of newly acidified organelles during autophagy enabled by reaction-based BODIPY dyes.

Liu Hanzhuang H   Song Wenting W   Gröninger Delia D   Zhang Lei L   Lu Yinghong Y   Chan Kin Shing KS   Zhou Zhikuan Z   Rurack Knut K   Shen Zhen Z  

Communications biology 20191128


Real-time monitoring of newly acidified organelles during autophagy in living cells is highly desirable for a better understanding of intracellular degradative processes. Herein, we describe a reaction-based boron dipyrromethene (BODIPY) dye containing strongly electron-withdrawing diethyl 2-cyanoacrylate groups at the α-positions. The probe exhibits intense red fluorescence in acidic organelles or the acidified cytosol while exhibiting negligible fluorescence in other regions of the cell. The u  ...[more]

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