Real-Time Pyruvate Chemical Conversion Monitoring Enabled by PHIP.

ABSTRACT: In recent years, parahydrogen-induced polarization side arm hydrogenation (PHIP-SAH) has been applied to hyperpolarize [1-¹³C]pyruvate and map its metabolic conversion to [1-¹³C]lactate in cancer cells. Developing on our recent MINERVA pulse sequence protocol, in which we have achieved 27% [1-¹³C]pyruvate carbon polarization, we demonstrate the hyperpolarization of [1,2-¹³C]pyruvate (∼7% polarization on each ¹³C spin) via PHIP-SAH. By altering a single parameter in the pulse sequence, MINERVA enables the signal enhancement of C1 and/or C2 in [1,2-¹³C]pyruvate with the opposite phase, which allows for the simultaneous monitoring of different chemical reactions with enhanced spectral contrast or for the same reaction via different carbon sites. We first demonstrate the ability to monitor the same enzymatic pyruvate to lactate conversion at 7T in an aqueous solution, in vitro, and in-cell (HeLa cells) via different carbon sites. In a second set of experiments, we use the C1 and C2 carbon positions as spectral probes for simultaneous chemical reactions: the production of acetate, carbon dioxide, bicarbonate, and carbonate by reacting [1,2-¹³C]pyruvate with H₂O₂ at a high temperature (55 °C). Importantly, we detect and characterize the intermediate 2-hydroperoxy-2-hydroxypropanoate in real time and at high temperature.

SUBMITTER: Stevanato G 

PROVIDER: S-EPMC10021011 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature