An exploration of the gut and environmental resistome in a community in northern Vietnam in relation to antibiotic use.

ABSTRACT: Background:Antibiotic resistance is a major global public health threat. Antibiotic use can directly impact the antibiotic resistant genes (ARGs) profile of the human intestinal microbiome and consequently the environment through shedding. Methods:We determined the resistome of human feces, animal stools, human food and environmental (rain, well, and irrigative water) samples (n =?304) in 40 households within a community cohort and related the data to antibiotic consumption. Metagenomic DNA was isolated and qPCR was used to determine presence of mobile colistin resistance (mcr) genes, genes encoding extended-spectrum ?-lactamases (ESBL), carbapenemases and quinolone resistance genes. Results:Nearly 40 % (39.5%, 120/304) of samples contained ESBL genes (most frequent were CTX-M-9 (23.7% [72/304]), CTX-M-1 (18.8% [57/304]). Quinolone resistance genes (qnrS) were detected in all human and 91% (41/45) of animal stool samples. Mcr-1 and mcr-3 were predominantly detected in human feces at 88% (82/93) and 55% (51/93) and animal feces at 93% (42/45) and 51% (23/45), respectively. Mcr-2, mrc-4 and mcr-5 were not detected in human feces, and only sporadically (

SUBMITTER: Bich VTN 

PROVIDER: S-EPMC6883630 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature