Ontology highlight
ABSTRACT:
SUBMITTER: Jack BR
PROVIDER: S-EPMC6884357 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Jack Benjamin R BR Leonard Sean P SP Mishler Dennis M DM Renda Brian A BA Leon Dacia D Suárez Gabriel A GA Barrick Jeffrey E JE
ACS synthetic biology 20150701 8
Unwanted evolution can rapidly degrade the performance of genetically engineered circuits and metabolic pathways installed in living organisms. We created the Evolutionary Failure Mode (EFM) Calculator to computationally detect common sources of genetic instability in an input DNA sequence. It predicts two types of mutational hotspots: deletions mediated by homologous recombination and indels caused by replication slippage on simple sequence repeats. We tested the performance of our algorithm on ...[more]