Unknown

Dataset Information

0

An Anti-BCMA RNA Aptamer for miRNA Intracellular Delivery.


ABSTRACT: B cell maturation antigen is highly expressed on malignant plasma cells in human multiple myeloma and has recently emerged as a very promising target for therapeutic interventions. Nucleic-acid-based aptamers are small oligonucleotides with high selective targeting properties and functional advantages over monoclonal antibodies, as both diagnostic and therapeutic tools. Here, we describe the generation of the first-ever-described nuclease resistant RNA aptamer selectively binding to B cell maturation antigen. We adopted a modified cell-based systematic evolution of ligands by exponential enrichment approach allowing the enrichment for internalizing aptamers. The selected 2'Fluoro-Pyrimidine modified aptamer, named apt69.T, effectively and selectively bound B cell maturation antigen-expressing myeloma cells with rapid and efficient internalization. Interestingly, apt69.T inhibited APRIL-dependent nuclear factor ?B (NF-?B) pathway in vitro. Moreover, the aptamer was conjugated to microRNA-137 (miR-137) and anti-miR-222, demonstrating high potential against tumor cells. In conclusion, apt69.T is a novel tool suitable for direct targeting and delivery of therapeutics to B cell maturation antigen-expressing myeloma cells.

SUBMITTER: Catuogno S 

PROVIDER: S-EPMC6889555 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

An Anti-BCMA RNA Aptamer for miRNA Intracellular Delivery.

Catuogno Silvia S   Di Martino Maria Teresa MT   Nuzzo Silvia S   Esposito Carla Lucia CL   Tassone Pierfrancesco P   de Franciscis Vittorio V  

Molecular therapy. Nucleic acids 20191028


B cell maturation antigen is highly expressed on malignant plasma cells in human multiple myeloma and has recently emerged as a very promising target for therapeutic interventions. Nucleic-acid-based aptamers are small oligonucleotides with high selective targeting properties and functional advantages over monoclonal antibodies, as both diagnostic and therapeutic tools. Here, we describe the generation of the first-ever-described nuclease resistant RNA aptamer selectively binding to B cell matur  ...[more]

Similar Datasets

| S-EPMC4723066 | biostudies-other
| S-EPMC6542267 | biostudies-literature
| S-EPMC7994153 | biostudies-literature
| S-EPMC3412061 | biostudies-literature
| S-EPMC6612664 | biostudies-literature
| S-EPMC4089008 | biostudies-other
| S-EPMC3107903 | biostudies-literature
| S-EPMC2275087 | biostudies-literature
| S-EPMC3553944 | biostudies-literature
| S-EPMC7076674 | biostudies-literature