Ontology highlight
ABSTRACT:
SUBMITTER: Ogawa J
PROVIDER: S-EPMC6892608 | biostudies-literature | 2018 Apr
REPOSITORIES: biostudies-literature
Ogawa Junko J Pao Gerald M GM Shokhirev Maxim N MN Verma Inder M IM
Cell reports 20180401 4
We have developed a cancer model of gliomas in human cerebral organoids that allows direct observation of tumor initiation as well as continuous microscopic observations. We used CRISPR/Cas9 technology to target an HRas<sup>G12V</sup>-IRES-tdTomato construct by homologous recombination into the TP53 locus. Results show that transformed cells rapidly become invasive and destroy surrounding organoid structures, overwhelming the entire organoid. Tumor cells in the organoids can be orthotopically xe ...[more]