Dataset Information

Circadian rhythms in Per1, PER2 and Ca²⁺ of a solitary SCN neuron cultured on a microisland.

ABSTRACT: Circadian rhythms in Per1, PER2 expression and intracellular Ca²⁺ were measured from a solitary SCN neuron or glial cell which was physically isolated from other cells. Dispersed cells were cultured on a platform of microisland (100-200 μm in diameter) in a culture dish. Significant circadian rhythms were detected in 57.1% for Per1 and 70.0% for PER2 expression. When two neurons were located on the same island, the circadian rhythms showed desynchronization, indicating a lack of oscillatory coupling. Circadian rhythms were also detected in intracellular Ca²⁺ of solitary SCN neurons. The ratio of circadian positive neurons was significantly larger without co-habitant of glial cells (84.4%) than with it (25.0%). A relatively large fraction of SCN neurons generates the intrinsic circadian oscillation without neural or humoral networks. In addition, glial cells seem to interrupt the expression of the circadian rhythmicity of intracellular Ca²⁺ under these conditions.

SUBMITTER: Hirata Y

PROVIDER: S-EPMC6892917 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Circadian rhythms in Per1, PER2 and Ca<sup>2+</sup> of a solitary SCN neuron cultured on a microisland.

Hirata Yoshihiro Y Enoki Ryosuke R Kuribayashi-Shigetomi Kaori K Oda Yoshiaki Y Honma Sato S Honma Ken-Ichi KI

Scientific reports 20191204 1

Circadian rhythms in Per1, PER2 expression and intracellular Ca<sup>2+</sup> were measured from a solitary SCN neuron or glial cell which was physically isolated from other cells. Dispersed cells were cultured on a platform of microisland (100-200 μm in diameter) in a culture dish. Significant circadian rhythms were detected in 57.1% for Per1 and 70.0% for PER2 expression. When two neurons were located on the same island, the circadian rhythms showed desynchronization, indicating a lack of oscil ...[more]

PMID: 31797953

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Project description:Human and animal studies demonstrate that short sleep or poor sleep quality, e.g. in night shift workers, promote the development of obesity and diabetes. Effects of sleep disruption on glucose homeostasis and liver physiology are well documented. However, changes in adipokine levels after sleep disruption suggest that adipocytes might be another important peripheral target of sleep. Circadian clocks regulate metabolic homeostasis and clock disruption can result in obesity and the metabolic syndrome. The finding that sleep and clock disruption have very similar metabolic effects prompted us to ask whether the circadian clock machinery may mediate the metabolic consequences of sleep disruption. To test this we analyzed energy homeostasis and adipocyte transcriptome regulation in a mouse model of shift work, in which we prevented mice from sleeping during the first six hours of their normal inactive phase for five consecutive days (timed sleep restriction--TSR). We compared the effects of TSR between wild-type and Per1/2 double mutant mice with the prediction that the absence of a circadian clock in Per1/2 mutants would result in a blunted metabolic response to TSR. In wild-types, TSR induces significant transcriptional reprogramming of white adipose tissue, suggestive of increased lipogenesis, together with increased secretion of the adipokine leptin and increased food intake, hallmarks of obesity and associated leptin resistance. Some of these changes persist for at least one week after the end of TSR, indicating that even short episodes of sleep disruption can induce prolonged physiological impairments. In contrast, Per1/2 deficient mice show blunted effects of TSR on food intake, leptin levels and adipose transcription. We conclude that the absence of a functional clock in Per1/2 double mutants protects these mice from TSR-induced metabolic reprogramming, suggesting a role of the circadian timing system in regulating the physiological effects of sleep disruption.

Dataset Information

Circadian rhythms in Per1, PER2 and Ca²⁺ of a solitary SCN neuron cultured on a microisland.

Publications

Circadian rhythms in Per1, PER2 and Ca<sup>2+</sup> of a solitary SCN neuron cultured on a microisland.

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Dataset Information

Circadian rhythms in Per1, PER2 and Ca2+ of a solitary SCN neuron cultured on a microisland.

Publications

Circadian rhythms in Per1, PER2 and Ca<sup>2+</sup> of a solitary SCN neuron cultured on a microisland.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Circadian rhythms in Per1, PER2 and Ca²⁺ of a solitary SCN neuron cultured on a microisland.