Ontology highlight
ABSTRACT:
SUBMITTER: Querques I
PROVIDER: S-EPMC6894935 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
Querques Irma I Mades Andreas A Zuliani Cecilia C Miskey Csaba C Alb Miriam M Grueso Esther E Machwirth Markus M Rausch Tobias T Einsele Hermann H Ivics Zoltán Z Hudecek Michael M Barabas Orsolya O
Nature biotechnology 20191104 12
The Sleeping Beauty (SB) transposon system is an efficient non-viral gene transfer tool in mammalian cells, but its broad use has been hampered by uncontrolled transposase gene activity from DNA vectors, posing a risk of genome instability, and by the inability to use the transposase protein directly. In this study, we used rational protein design based on the crystal structure of the hyperactive SB100X variant to create an SB transposase (high-solubility SB, hsSB) with enhanced solubility and s ...[more]