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Integration of Quantitative Trait Loci Mapping and Expression Profiling Analysis to Identify Genes Potentially Involved in Ramie Fiber Lignin Biosynthesis.

ABSTRACT: Ramie fibers, one of the most important natural fibers in China, are mainly composed of lignin, cellulose, and hemicellulose. As the high lignin content in the fibers results in a prickly texture, the lignin content is deemed to be an important trait of the fiber quality. In this study, the genetic basis of the fiber lignin content was evaluated, resulting in the identification of five quantitative trait loci (QTLs). Three genes, whole_GLEAN_10021050, whole_GLEAN_10026962, and whole_GLEAN_10009464 that were identified on the QTL regions of qLC7, qLC10, and qLC13, respectively, were found to be homologs of the Arabidopsis lignin biosynthetic genes. Moreover, all three genes displayed differential expression in the barks located in the top and middle parts of the stem, where lignin was not being synthesized and where it was being biosynthesized, respectively. Sequence comparison found that these three genes had wide variations in their coding sequences (CDSs) and putative promoter regions between the two parents, especially the MYB gene whole_GLEAN_10021050, whose protein had insertions/deletions of five amino acids and substitutions of two amino acids in the conserved domain. This evidence indicates that these three genes are potentially involved in lignin biosynthesis in ramie fibers. The QTLs identified from this study provide a basis for the improvement of lignin content and fiber quality in ramie breeding. The characterization of the three candidate genes here will be helpful for the future clarification of their functions in ramie.

SUBMITTER: Chen J

PROVIDER: S-EPMC6896145 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Integration of Quantitative Trait Loci Mapping and Expression Profiling Analysis to Identify Genes Potentially Involved in Ramie Fiber Lignin Biosynthesis.

Chen Jianrong J Rao Jing J Wang Yanzhou Y Zeng Zheng Z Liu Fang F Tang Yinghong Y Chen Xiaorong X Liu Chan C Liu Touming T

Genes 20191024 11

Ramie fibers, one of the most important natural fibers in China, are mainly composed of lignin, cellulose, and hemicellulose. As the high lignin content in the fibers results in a prickly texture, the lignin content is deemed to be an important trait of the fiber quality. In this study, the genetic basis of the fiber lignin content was evaluated, resulting in the identification of five quantitative trait loci (QTLs). Three genes, whole_GLEAN_10021050, whole_GLEAN_10026962, and w ...[more]

PMID: 31653111

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Project description:Carbon fiber reinforced composites have exceptional potential to play a key role in the materials world of our future. However, their success undoubtedly depends on the extent they can contribute to advance a global sustainability objective. Utilizing polymers in these composites that can be potentially derived from biomasses would be certainly vital for next-generation manufacturing practices. Nevertheless, deep understanding and tailoring fiber-matrix interactions are crucial issues in order to design carbon fiber reinforced sustainable resource-based biocomposites. In this study, cellulose derivatives (cellulose propionate and cellulose acetate butyrate) are utilized as model polymer matrices that can be potentially fabricated from biomasses, and the mechanical properties of the prepared short carbon fiber reinforced composites are engineered by means of a functional biobased lignin coating on the fiber surface. Furthermore, polyamide 6 based composites are also prepared, the monomer of this polymer could be obtained using C6 sugars derived from lignocellulosic biomasses in the future (through 5-hydroxymethylfurfural). Lignin was successfully immobilized on the carbon fiber surface via an industrially scalable benign epoxidation reaction. The surface modification had a beneficial impact on the mechanical properties of cellulose propionate and polyamide 6 composites. Furthermore, our results also revealed that cellulose-based matrices are highly sensitive to the presence of rigid fiber segments that restrict polymer chain movements and facilitate stress development. It follows that the physicochemical properties of the cellulosic matrices (molecular weight, crystallinity), associated with polymer chain mobility, might need to be carefully considered when designing these composites. At the same time, polyamide 6 showed excellent ability to accommodate short carbon fibers without leading to a largely brittle material, in this case, a maximum tensile strength of ~136 MPa was obtained at 20 wt% fiber loading. These results were further contrasted with that of a petroleum-based polypropylene matrix exhibiting inferior mechanical properties. Our study clearly indicates that carbon fiber reinforced polymers derived and designed using biomass-derived resources can be promising green materials for a sustainable future.

Dataset Information

Integration of Quantitative Trait Loci Mapping and Expression Profiling Analysis to Identify Genes Potentially Involved in Ramie Fiber Lignin Biosynthesis.

Publications

Integration of Quantitative Trait Loci Mapping and Expression Profiling Analysis to Identify Genes Potentially Involved in Ramie Fiber Lignin Biosynthesis.

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