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Polyvinylalcohol-carbazate (PVAC) reduces red blood cell hemolysis.

ABSTRACT: BACKGROUND AND OBJECTIVES:The objective of this study was to investigate whether a soluble polymer and aldehyde-scavenger, polyvinylalcohol-carbazate (PVAC), can inhibit hemolysis in the storage of red blood cells (RBC). STUDY DESIGN AND METHODS:The effect of PVAC was assessed over a wide range of concentrations, using absorption spectroscopy to evaluate the level of hemolysis. Moreover, osmotic stability and aldehyde-scavenging potential of RBC were assessed after storage in PVAC. RESULTS:After test tube storage for two weeks, red blood cell hemolysis was lower with PVAC compared to controls (mean difference 23%, 95% CI 16-29%, p < 0.001). A higher level of hemolysis led to a pronounced effect with PVAC. RBC stored in PVAC improved both the binding of free aldehydes (p <0.001) and the osmotic stability (p = 0.0036). CONCLUSION:Erythrocytes stored with PVAC showed less hemolysis, which might be explained by the ability of PVACs to stabilize the cell membrane and decrease oxidative injury.

SUBMITTER: Sellberg F 

PROVIDER: S-EPMC6897416 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Polyvinylalcohol-carbazate (PVAC) reduces red blood cell hemolysis.

Sellberg Felix F   Fredriksson Fanny F   Engstrand Thomas T   Bowden Tim Melander TM   Nilsson Bo B   Hong Jaan J   Knutson Folke F   Berglund David D  

PloS one 20191206 12

<h4>Background and objectives</h4>The objective of this study was to investigate whether a soluble polymer and aldehyde-scavenger, polyvinylalcohol-carbazate (PVAC), can inhibit hemolysis in the storage of red blood cells (RBC).<h4>Study design and methods</h4>The effect of PVAC was assessed over a wide range of concentrations, using absorption spectroscopy to evaluate the level of hemolysis. Moreover, osmotic stability and aldehyde-scavenging potential of RBC were assessed after storage in PVAC  ...[more]

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