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Influence of the regulatory peptide galanin on cytokine expression in human monocytes.

ABSTRACT: Current research into neuropeptides is bringing to light many remarkable functions of these endocrine/neurocrine/paracrine factors, such as their roles in modulating immune responses. Galanin is a neuropeptide expressed in both neural and non-neural tissues and exerts its effects through three G protein-coupled receptors, GAL1,2,3 -R. It has been demonstrated that galanin has modulatory effects on immune cells, including neutrophils and natural killer cells. Because monocytes express GAL2 -R, and therefore are expected to be a target of galanin, we analyzed the effect of galanin on the expression of cytokines and chemokines by monocytes. Galanin increased the expression of IL-1? up to 1.5-fold, TNF-?, IL-10, IL-18, and CCL3 up to twofold, and CXCL8 up to fourfold in nonactivated monocytes, but had no major effect on activated monocytes. A cross-correlation analysis of cytokine expression profiles, irrespective of the activation status of the monocytes, revealed that galanin changed the cross-correlation of the expression of certain cytokines. Galanin abolished several significant correlations in IFN-?-stimulated monocytes. For example, treatment with 10 nM galanin changed the Spearman's rank coefficient of IL-18 and CXCL8 from 0.622 (P ? 0.01) to 0.126. These results further emphasize the importance of neuroregulatory peptides, such as galanin and their therapeutic potential to treat inflammatory diseases.

SUBMITTER: Ramspacher A

PROVIDER: S-EPMC6899851 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Influence of the regulatory peptide galanin on cytokine expression in human monocytes.

Ramspacher Andrea A Neudert Magdalena M Koller Andreas A Schlager Sandra S Kofler Barbara B Brunner Susanne Maria SM

Annals of the New York Academy of Sciences 20190510 1

Current research into neuropeptides is bringing to light many remarkable functions of these endocrine/neurocrine/paracrine factors, such as their roles in modulating immune responses. Galanin is a neuropeptide expressed in both neural and non-neural tissues and exerts its effects through three G protein-coupled receptors, GAL<sub>1,2,3</sub> -R. It has been demonstrated that galanin has modulatory effects on immune cells, including neutrophils and natural killer cells. Because monocytes express ...[more]

PMID: 31074091

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Influence of the regulatory peptide galanin on cytokine expression in human monocytes.

Publications

Influence of the regulatory peptide galanin on cytokine expression in human monocytes.

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