Ontology highlight
ABSTRACT:
SUBMITTER: Xu X
PROVIDER: S-EPMC6900072 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Xu Xiaomeng X Barreiro Karina K Musante Luca L Kretz Oliver O Lin Hanfei H Zou Hequn H Huber Tobias B TB Holthofer Harry H
Proteomics. Clinical applications 20190908 6
<h4>Purpose</h4>Urinary extracellular vesicles (uEVs) are a novel source of biomarkers. However, urinary Tamm-Horsfall Protein (THP; uromodulin) interferes with all vesicle isolation attempts, precipitates with normal urinary proteins, thus, representing an unwanted "contaminant" in urinary assays. Thus, the aim is to develop a simple method to manage THP efficiently.<h4>Experimental design</h4>The uEVs are isolated by hydrostatic filtration dialysis (HFD) and treated with a defined solution of ...[more]