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TcellSubC: An Atlas of the Subcellular Proteome of Human T Cells.

ABSTRACT: We have curated an in-depth subcellular proteomic map of primary human CD4+ T cells, divided into cytosolic, nuclear and membrane fractions generated by an optimized fractionation and HiRIEF-LC-MS/MS workflow for limited amounts of primary cells. The subcellular proteome of T cells was mapped under steady state conditions, as well as upon 15 min and 1 h of T cell receptor (TCR) stimulation, respectively. We quantified the subcellular distribution of 6,572 proteins and identified a subset of 237 potentially translocating proteins, including both well-known examples and novel ones. Microscopic validation confirmed the localization of selected proteins with previously known and unknown localization, respectively. We further provide the data in an easy-to-use web platform to facilitate re-use, as the data can be relevant for basic research as well as for clinical exploitation of T cells as therapeutic targets.

SUBMITTER: Joshi RN

PROVIDER: S-EPMC6902019 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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TcellSubC: An Atlas of the Subcellular Proteome of Human T Cells.

Joshi Rubin Narayan RN Stadler Charlotte C Lehmann Robert R Lehtiö Janne J Tegnér Jesper J Schmidt Angelika A Vesterlund Mattias M

Frontiers in immunology 20191126

We have curated an in-depth subcellular proteomic map of primary human CD4+ T cells, divided into cytosolic, nuclear and membrane fractions generated by an optimized fractionation and HiRIEF-LC-MS/MS workflow for limited amounts of primary cells. The subcellular proteome of T cells was mapped under steady state conditions, as well as upon 15 min and 1 h of T cell receptor (TCR) stimulation, respectively. We quantified the subcellular distribution of 6,572 proteins and identified a subset of 237 ...[more]

PMID: 31849937

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